Polb (NM_011130) Mouse Untagged Clone

CAT#: MC209200

Polb (untagged) - Mouse polymerase (DNA directed), beta (Polb), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Polb
• Synonyms: A430088C08Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209200 representing NM_011130
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCAAACGCAAGGCGCCGCAGGAGACCCTCAACGGCGGCATCACGGACATGCTCGTGGAACTCGCAA
  ACTTTGAGAAGAACGTGAGCCAGGCGATCCACAAGTACAATGCGTACAGAAAAGCGGCATCTGTGATAGC
  CAAGTACCCACACAAAATCAAGAGCGGAGCGGAAGCTAAGAAACTGCCAGGAGTAGGAACAAAAATTGCT
  GAAAAGATTGATGAATTTTTAGCAACTGGAAAATTGCGTAAACTGGAAAAGATTCGTCAAGATGATACAA
  GTTCATCCATCAATTTCCTGACTCGAGTTACTGGCATTGGACCATCTGCTGCAAGGAAGTTTGTAGATGA
  AGGAATTAAGACATTAGAAGATCTCAGGAAAAATGAAGATAAACTGAACCATCATCAACGAATTGGGCTG
  AAATATTTCGAGGACTTTGAAAAGAGAATTCCTCGTGAGGAAATGCTGCAGATGCAGGATATTGTACTTA
  ATGAAATTAAAAAAGTGGACTCTGAGTACATTGCTACAGTCTGTGGCAGTTTCAGAAGAGGCGCAGAGTC
  GAGTGGAGACATGGATGTCCTGCTGACCCACCCAAACTTCACGTCAGAATCCAGCAAACAGCCAAAGTTG
  TTACATCGTGTTGTGGAACAGTTACAAAAAGTCCATTTCATTACAGATACTCTGTCAAAGGGTGAAACAA
  AGTTCATGGGTGTTTGCCAGCTTCCCAGCGAGAAGGATGGAAAGGAATATCCACACAGGAGAATCGATAT
  CAGGTTGATCCCCAAAGATCAGTACTACTGTGGTGTTCTCTACTTCACTGGGAGTGACATCTTTAATAAG
  AACATGAGAGCGCACGCCCTGGAAAAGGGCTTCACAATCAATGAGTACACCATCCGCCCCCTGGGGGTCA
  CTGGAGTCGCTGGGGAGCCCCTTCCTGTGGACAGTGAGCAGGACATTTTTGATTACATCCAGTGGCGCTA
  CCGGGAGCCCAAGGATAGAAGTGAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011130
• Insert Size: 1008 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011130.2, NP_035260.1
• RefSeq Size: 1227 bp
• RefSeq ORF: 1008 bp
• Locus ID: 18970
• UniProt ID: Q8K409
• Cytogenetics: 8 11.42 cM
• Gene Summary: Repair polymerase that plays a key role in base-excision repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases (By similarity).[UniProtKB/Swiss-Prot Function]