Prkacb (NM_011100) Mouse Untagged Clone

CAT#: MC209176

Prkacb (untagged) - Mouse protein kinase, cAMP dependent, catalytic, beta (Prkacb), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Prkacb
• Synonyms: CbPKA; Pkacb
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209176 representing NM_011100
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC
  GCCC
  
  ATGGGGAACACTGCGATCGCCAAGAAAGGCAGCGAAGTGGAGAGCGTGAAAGAGTTTCTAGCCAAAGCCA
  AAGAAGACTTTCTGAGGAAATGGGAGAACCCTCCCCCGAGTAATGCTGGGCTTGAGGATTTTGAGAGGAA
  GAAAACCCTCGGGACGGGTTCCTTTGGAAGAGTCATGTTGGTGAAGCATAAAGCCACTGAGCAGTACTAC
  GCCATGAAGATCTTAGACAAGCAGAAGGTTGTTAAGCTGAAGCAAATAGAGCACACTCTGAATGAGAAGA
  GAATCCTGCAGGCCGTGGAGTTCCCGTTCCTTGTGCGGCTGGAGTACTCTTTTAAGGATAATTCTAATTT
  ATACATGGTTATGGAATACGTCCCTGGGGGAGAGATGTTCTCACATCTGAGAAGAATTGGAAGGTTCAGT
  GAGCCCCACGCCCGTTTCTATGCAGCCCAGATTGTGCTAACATTTGAGTACCTTCATTCCCTCGACCTCA
  TCTACAGAGATCTCAAGCCGGAAAACCTCTTAATTGACCACCAGGGTTACATCCAGGTCACAGATTTCGG
  GTTCGCCAAAAGAGTCAAGGGCAGGACATGGACATTGTGTGGCACCCCAGAGTACCTGGCCCCGGAGATC
  ATCCTCAGCAAGGGTTACAATAAGGCGGTGGACTGGTGGGCACTGGGCGTGCTGATCTATGAGATGGCTG
  CTGGCTACCCTCCATTCTTTGCTGACCAGCCAATTCAGATCTATGAGAAGATTGTCTCTGGAAAGGTCCG
  GTTCCCATCACACTTCAGCTCCGATCTCAAGGACCTTCTGCGGAACCTGCTGCAGGTGGATCTGACAAAG
  CGATTCGGGAACCTGAAGAACGGCGTGAGTGACATAAAGACCCACAAGTGGTTTGCCACAACTGACTGGA
  TTGCTATTTATCAGAGAAAGGTTGAGGCTCCATTCATACCAAAGTTCAGAGGCTCTGGCGATACCAGCAA
  CTTCGATGACTATGAAGAAGAAGAAATCCGTGTGTCTATAACAGAAAAATGTGGAAAGGAATTTTGTGAA
  TTTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: AscI-MluI
• ACCN: NM_011100
• Insert Size: 1056 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011100.4, NP_035230.1
• RefSeq Size: 4341 bp
• RefSeq ORF: 1056 bp
• Locus ID: 18749
• UniProt ID: P68181
• Cytogenetics: 3 H2
• Gene Summary: Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux (PubMed:9368018). Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates GPKOW which regulates its ability to bind RNA (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes isoform 1.