Prkacb (NM_001164198) Mouse Untagged Clone

CAT#: MC209174

Prkacb (untagged) - Mouse protein kinase, cAMP dependent, catalytic, beta (Prkacb), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Prkacb
• Synonyms: CbPKA; Pkacb
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209174 representing NM_001164198
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGTGAAAGAGTTTCTAGCCAAAGCCAAAGAAGACTTTCTGAGGAAATGGGAGAACCCTCCCCCGA
  GTAATGCTGGGCTTGAGGATTTTGAGAGGAAGAAAACCCTCGGGACGGGTTCCTTTGGAAGAGTCATGTT
  GGTGAAGCATAAAGCCACTGAGCAGTACTACGCCATGAAGATCTTAGACAAGCAGAAGGTTGTTAAGCTG
  AAGCAAATAGAGCACACTCTGAATGAGAAGAGAATCCTGCAGGCCGTGGAGTTCCCGTTCCTTGTGCGGC
  TGGAGTACTCTTTTAAGGATAATTCTAATTTATACATGGTTATGGAATACGTCCCTGGGGGAGAGATGTT
  CTCACATCTGAGAAGAATTGGAAGGTTCAGTGAGCCCCACGCCCGTTTCTATGCAGCCCAGATTGTGCTA
  ACATTTGAGTACCTTCATTCCCTCGACCTCATCTACAGAGATCTCAAGCCGGAAAACCTCTTAATTGACC
  ACCAGGGTTACATCCAGGTCACAGATTTCGGGTTCGCCAAAAGAGTCAAGGGCAGGACATGGACATTGTG
  TGGCACCCCAGAGTACCTGGCCCCGGAGATCATCCTCAGCAAGGGTTACAATAAGGCGGTGGACTGGTGG
  GCACTGGGCGTGCTGATCTATGAGATGGCTGCTGGCTACCCTCCATTCTTTGCTGACCAGCCAATTCAGA
  TCTATGAGAAGATTGTCTCTGGAAAGGTCCGGTTCCCATCACACTTCAGCTCCGATCTCAAGGACCTTCT
  GCGGAACCTGCTGCAGGTGGATCTGACAAAGCGATTCGGGAACCTGAAGAACGGCGTGAGTGACATAAAG
  ACCCACAAGTGGTTTGCCACAACTGACTGGATTGCTATTTATCAGAGAAAGGTTGAGGCTCCATTCATAC
  CAAAGTTCAGAGGCTCTGGCGATACCAGCAACTTCGATGACTATGAAGAAGAAGAAATCCGTGTGTCTAT
  AACAGAAAAATGTGGAAAGGAATTTTGTGAATTTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001164198
• Insert Size: 1017 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164198.1, NP_001157670.1
• RefSeq Size: 4337 bp
• RefSeq ORF: 1017 bp
• Locus ID: 18749
• UniProt ID: P68181
• Cytogenetics: 3 H2
• Gene Summary: Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux (PubMed:9368018). Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates GPKOW which regulates its ability to bind RNA (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternative exon at the 5' end compared to variant 1. The resulting isoform (2) has a distinct and shorter N-terminus, as compared to isoform 1.