Pip4k2a (NM_008845) Mouse Untagged Clone

CAT#: MC209168

Pip4k2a (untagged) - Mouse phosphatidylinositol-5-phosphate 4-kinase, type II, alpha (Pip4k2a), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pip4k2a
• Synonyms: AW742916; Pip5k2a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209168 representing NM_008845
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACCCCTGGCAACCTGGGGTCCTCCGTCCTGGCGAGCAAGACCAAGACGAAGAAGAAGCACTTCG
  TGGCTCAGAAAGTGAAGCTGTTCCGGGCCAGCGACCCGCTGCTCAGCGTGCTCATGTGGGGGGTCAACCA
  TTCGATCAATGAACTGAGCCACGTTCAAATCCCTGTCATGTTGATGCCTGATGACTTCAAAGCCTACTCA
  AAGATAAAGGTTGACAACCACCTTTTTAACAAGGAAAACATGCCGAGCCATTTCAAGTTTAAGGAATACT
  GCCCAATGGTCTTCCGGAATCTGCGGGAGAGGTTTGGAATCGACGACCAAGATTTCCAGAATTCCTTGAC
  CAGAAGTGCACCCCTTCCCAATGACTCCCAGGCTCGCAGCGGGGCTCGGTTTCACACGTCTTATGATAAA
  AGATACGTCATCAAGACCATTACCAGTGAGGACGTGGCAGAGATGCACAACATCCTGAAGAAGTACCACC
  AGTATATAGTGGAATGTCATGGGGTCACACTTCTTCCTCAGTTCTTGGGAATGTACCGGCTTAATGTCGA
  TGGAGTGGAAATATATGTGATTGTTACAAGGAATGTGTTCAGCCACCGGCTATCTGTATATAGGAAATAC
  GACTTAAAGGGCTCGACAGTGGCTAGAGAAGCTAGTGATAAAGAAAAGGCCAAAGAGCTGCCAACTTTAA
  AGGATAATGACTTCATCAATGAGGGCCAAAAGATCTATATTGATGACAACAACAAAAAGATATTCCTAGA
  AAAACTGAAAAAGGATGTCGAGTTCCTTGCACAGTTAAAACTCATGGACTACAGCCTCCTGGTGGGGATT
  CACGATGTGGAGCGAGCAGAGCAGGAGGAGGTGGAGTGTGAGGAGAACGATGGGGAAGAAGAAGGAGAGA
  GTGACAGCACCCACCCGATCGGGACGCCACCAGACAGTCCCGGAAATACACTCAACAGCTCACCACCCTT
  GGCTCCTGGAGAGTTTGATCCAAACATTGATGTTTATGCAATTAAATGCCATGAGAATGCGCCTAGAAAA
  GAGGTATACTTCATGGCGATTATTGACATCCTTACTCATTACGATGCAAAAAAGAAAGCTGCCCACGCTG
  CAAAAACTGTTAAACATGGCGCTGGAGCTGAGATCTCGACTGTGAACCCAGAGCAGTATTCCAAGCGCTT
  TTTGGACTTTATTGGGCACATCTTGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008845
• Insert Size: 1218 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008845.4, NP_032871.3
• RefSeq Size: 3471 bp
• RefSeq ORF: 1218 bp
• Locus ID: 18718
• UniProt ID: O70172
• Cytogenetics: 2 A3
• Gene Summary: Catalyzes the phosphorylation of phosphatidylinositol 5-phosphate (PtdIns5P) on the fourth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). May exert its function by regulating the levels of PtdIns5P, which functions in the cytosol by increasing AKT activity and in the nucleus signals through ING2. May regulate the pool of cytosolic PtdIns5P in response to the activation of tyrosine phosphorylation. May negatively regulate insulin-stimulated glucose uptake by lowering the levels of PtdIns5P (By similarity). May be involved in thrombopoiesis and the terminal maturation of megakaryocytes and regulation of their size.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longest isoform (1).