Pdha2 (NM_008811) Mouse Untagged Clone

CAT#: MC209159

Pdha2 (untagged) - Mouse pyruvate dehydrogenase E1 alpha 2 (Pdha2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pdha2
• Synonyms: Pdhal
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209159 representing NM_008811
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGAAAATGCTGACCGCTGTGCTGTCTCACGTATTTTCGGGAATGGTCCAAAAGCCAGCTCTCAGAG
  GACTGCTGTCATCTCTGAAGTTCTCCAACGACGCCACCTGTGACATTAAGAAATGTGACCTGTACCGGCT
  GGAGGAGGGCCCACCGACCTCCACCGTGCTCACCCGAGCCGAGGCCCTCAAGTACTACCGGACCATGCAG
  GTAATTCGGCGCATGGAGTTGAAGGCCGACCAGCTGTATAAGCAGAAATTCATCCGTGGTTTCTGTCACC
  TGTGTGATGGGCAGGAAGCCTGCTGCGTGGGGCTGGAGGCAGGGATAAATCCCACGGATCACGTCATCAC
  GTCCTACCGGGCTCATGGCTTCTGCTACACGCGAGGACTGTCCGTGAAGTCCATTCTCGCCGAGCTGACT
  GGACGCAAAGGAGGCTGTGCTAAAGGCAAGGGAGGCTCCATGCACATGTACGGCAAGAACTTCTACGGTG
  GCAATGGCATTGTTGGGGCCCAGGTACCCCTGGGAGCTGGTGTGGCTTTTGCCTGTAAATACCTGAAGAA
  TGGTCAGGTCTGCTTGGCTTTGTACGGCGATGGTGCGGCTAACCAAGGGCAGGTATTCGAAGCATACAAT
  ATGTCAGCCTTGTGGAAATTACCCTGTGTTTTCATCTGTGAGAATAACCTCTATGGAATGGGAACCTCCA
  ACGAGAGATCAGCAGCCAGTACTGATTACCACAAGAAAGGTTTTATTATCCCCGGACTGAGGGTGAATGG
  GATGGATATTCTCTGTGTTCGGGAGGCAACCAAGTTTGCAGCTGATCACTGCAGATCTGGAAAGGGGCCC
  ATTGTGATGGAGCTGCAGACCTACCGTTATCATGGACACAGTATGAGCGACCCAGGGATCAGTTATCGTT
  CACGAGAAGAAGTTCATAACGTGAGAAGTAAGAGTGATCCTATAATGCTGCTCCGAGAGAGAATTATCAG
  CAACAACCTCAGCAATATTGAAGAATTGAAAGAAATTGATGCAGATGTGAAGAAAGAGGTGGAGGACGCA
  GCTCAGTTTGCTACGACTGATCCAGAACCAGCTGTGGAAGATATAGCCAATTACCTCTACCACCAAGATC
  CACCTTTTGAAGTCCGTGGTGCACATAAGTGGCTCAAGTATAAGTCCCACAGTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008811
• Insert Size: 1176 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008811.2, NP_032837.1
• RefSeq Size: 2352 bp
• RefSeq ORF: 1176 bp
• Locus ID: 18598
• UniProt ID: P35487
• Cytogenetics: 3 H1
• Gene Summary: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.[UniProtKB/Swiss-Prot Function]