Gpr143 (NM_010951) Mouse Untagged Clone

CAT#: MC209066

Gpr143 (untagged) - Mouse G protein-coupled receptor 143 (Gpr143), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gpr143
• Synonyms: Oa1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209066 representing NM_010951
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCCCCGCGCCTGGGAATTTTCTGCTGCCCTACGTGGGACGCAGCCACACAGCTGGTGCTAAGCT
  TCCAACCGCGGGTGTTCCATGCCCTGTGCCTGGGAAGCGGCACTCTCCGCCTGGTGCTTGGCCTCCTTCA
  GCTCCTATCAGGGCGTCGATCTGTTGGTCACAGGGCGCCTGCGACATCCCCAGCCGCCTCAGTCCACATC
  CTCCGTGCTGCCACTGCCTGTGACTTGCTTGGCTGCCTGGGAATCGTTATCAGGTCCACAGTGTGGATAG
  CCTACCCAGAGTTCATTGAAAACATTTCCAATGTGAATGCAACAGACATTTGGCCTGCTACTTTCTGTGT
  GGGGAGCGCAATGTGGATCCAGCTGTTGTACAGTGCCTGCTTCTGGTGGCTCTTTTGCTATGCAGTTGAT
  GTATACTTGGTGATCAGGAGATCGGCGGGACGGAGCACCATCCTGCTGTACCACATCATGGCCTGGGGCC
  TGGCTGTGCTGCTCTGTGTGGAGGGAGCAGTCATGCTCTACTACCCTTCTGTGTCCAGGTGTGAGAGGGG
  CCTGGACCATGCCATCCCCCATTATGTCACCACATACTTGCCACTTCTGCTTGTCCTGGTGGCCAACCCA
  ATCCTGTTTCACAAGACAGTGACTTCAGTGGCCTCTTTACTGAAAGGAAGAAAAGGTGTTTACACAGAGA
  ATGAGAGACTGATGGGGGCTGTGATCAAGACCCGTTTTTTCAAAATAATGCTGGTGTTAATTGCATGTTG
  GTTGTCCAATATCATCAATGAAAGTCTTTTGTTCTACCTTGAAATGCAACCAGATATCCATGGAGGCTCT
  CTGAAACGCATCCAGAATGCAGCTAGGACCACATGGTTTATAATGGGAATACTGAATCCAGCCCAAGGAC
  TTCTCTTGTCTCTGGCCTTCTATGGCTGGACAGGATGCAGCCTGGATGTCCATCCTCCCAAGATGGTGAT
  TCAGTGGGAAACAATGACTGCCTCTGCTGCTGAGGGCACGTACCAGACCCCTGTGCGTTCCTGTGTGCCC
  CATCAAAACCCCAGGAAGGTTGTATGTGTCGGGGGACATACTTCTGATGAGGTGCTGAGCATTTTGTCTG
  AAGATTCAGATGCCAGTACTGTTGAAATCCATACTGCAACTGGGTCCTGCAACATAAAGGAAGTTGACTC
  CATTTCCCAAGCCCAGGGGGAACTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_010951
• Insert Size: 1218 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010951.3, NP_035081.3
• RefSeq Size: 1651 bp
• RefSeq ORF: 1218 bp
• Locus ID: 18241
• UniProt ID: P70259
• Cytogenetics: X 68.46 cM
• Gene Summary: Receptor for tyrosine, L-DOPA and dopamine. After binding to L-DOPA, stimulates Ca(2+) influx into the cytoplasm, increases secretion of the neurotrophic factor SERPINF1 and relocalizes beta arrestin at the plasma membrane; this ligand-dependent signaling occurs through a G(q)-mediated pathway in melanocytic cells. Its activity is mediated by G proteins which activate the phosphoinositide signaling pathway. Plays also a role as an intracellular G protein-coupled receptor involved in melanosome biogenesis, organization and transport.[UniProtKB/Swiss-Prot Function]