Ntf3 (NM_001164034) Mouse Untagged Clone

CAT#: MC209060

Ntf3 (untagged) - Mouse neurotrophin 3 (Ntf3), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ntf3
• Synonyms: AI316846; AI835689; HDNF; NGF-2; NT; NT-; Nt3; Ntf-; Ntf-3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209060 representing NM_001164034
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTACTTCTGCCACGATCTTACAGGTGAACAAGGTGATGTCCATCTTGTTTTATGTGATATTTCTTG
  CTTATCTCCGTGGCATCCAAGGCAACAGCATGGATCAAAGGAGTTTGCCGGAAGACTCTCTCAATTCCCT
  CATCATCAAGCTGATCCAGGCGGATATCTTGAAAAACAAGCTTTCCAAACAGATGGTGGATGTTAAGGAA
  AATTACCAGAGCACCCTGCCCAAAGCAGAGGCACCCAGGGAACCAGAGCAGGGAGAGGCCACCAGGTCAG
  AGTTCCAGCCAATGATTGCAACGGACACAGAGCTACTACGGCAACAGAGACGCTACAATTCGCCCCGGGT
  CCTGCTGAGTGACAGCACCCCTTTGGAGCCCCCTCCCTTATACCTAATGGAGGATTATGTGGGCAACCCG
  GTGGTAGCCAATAGAACCTCACCACGGAGGAAACGCTATGCAGAACATAAGAGTCACCGAGGAGAGTACT
  CAGTGTGTGACAGTGAGAGCCTGTGGGTGACCGACAAGTCCTCAGCCATTGACATTCGGGGACACCAGGT
  CACAGTGCTGGGGGAGATCAAAACCGGTAACTCTCCTGTGAAACAATATTTTTATGAAACGAGATGTAAA
  GAAGCCAGGCCGGTCAAAAACGGTTGCAGGGGGATTGATGACAAACACTGGAACTCTCAGTGCAAAACTT
  CGCAAACCTATGTCCGAGCACTGACTTCAGAAAACAACAAACTCGTAGGCTGGCGCTGGATACGAATAGA
  CACTTCCTGTGTGTGTGCCTTGTCGAGAAAAATTGGAAGAACATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001164034
• Insert Size: 816 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164034.1, NP_001157506.1
• RefSeq Size: 1453 bp
• RefSeq ORF: 816 bp
• Locus ID: 18205
• UniProt ID: P20181
• Cytogenetics: 6 60.45 cM
• Gene Summary: This gene encodes a member of the neurotrophins that have a wide variety of functions in both neural and non-neural tissues. The encoded preproprotein undergoes proteolytic processing to generate a noncovalently linked homodimeric mature protein that can bind to the transmembrane receptor tyrosine kinases to initiate a series of signaling events. Mice lacking the encoded protein exhibit severe defects in the peripheral nervous system including a complete lack of spinal proprioceptive afferents and their peripheral sense organs. [provided by RefSeq, Sep 2016]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Both variants 1 and 2 encode the same isoform.