Ntf3 (NM_001164035) Mouse Untagged Clone

CAT#: MC209059

Ntf3 (untagged) - Mouse neurotrophin 3 (Ntf3), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ntf3
• Synonyms: AI316846; AI835689; HDNF; NGF-2; NT; NT-; Nt3; Ntf-; Ntf-3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209059 representing NM_001164035
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTACTTCTGCCACGATCTTACAGGTGAACAAGGTGATGTCCATCTTGTTTTATGTGATATTTCTTG
  CTTATCTCCGTGGCATCCAAGGCAACAGCATGGATCAAAGGAGTTTGCCGGAAGACTCTCTCAATTCCCT
  CATCATCAAGCTGATCCAGGCGGATATCTTGAAAAACAAGCTTTCCAAACAGATGGTGGATGTTAAGGAA
  AATTACCAGAGCACCCTGCCCAAAGCAGAGGCACCCAGGGAACCAGAGCAGGGAGAGGCCACCAGGTCAG
  AGTTCCAGCCAATGATTGCAACGGACACAGAGCTACTACGGCAACAGAGACGCTACAATTCGCCCCGGGT
  CCTGCTGAGTGACAGCACCCCTTTGGAGCCCCCTCCCTTATACCTAATGGAGGATTATGTGGGCAACCCG
  GTGGTAGCCAATAGAACCTCACCACGGAGGAAACGCTATGCAGAACATAAGAGTCACCGAGGAGAGTACT
  CAGTGTGTGACAGTGAGAGCCTGTGGGTGACCGACAAGTCCTCAGCCATTGACATTCGGGGACACCAGGT
  CACAGTGCTGGGGGAGATCAAAACCGGTAACTCTCCTGTGAAACAATATTTTTATGAAACGAGATGTAAA
  GAAGCCAGGCCGGTCAAAAACGGTTGCAGGGGGATTGATGACAAACACTGGAACTCTCAGTGCAAAACTT
  CGCAAACCTATGTCCGAGCACTGACTTCAGAAAACAACAAACTCGTAGGCTGGCGCTGGATACGAATAGA
  CACTTCCTGTGTGTGTGCCTTGTCGAGAAAAATTGGAAGAACATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001164035
• Insert Size: 816 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164035.1, NP_001157507.1
• RefSeq Size: 1368 bp
• RefSeq ORF: 816 bp
• Locus ID: 18205
• UniProt ID: P20181
• Cytogenetics: 6 60.45 cM
• Gene Summary: This gene encodes a member of the neurotrophins that have a wide variety of functions in both neural and non-neural tissues. The encoded preproprotein undergoes proteolytic processing to generate a noncovalently linked homodimeric mature protein that can bind to the transmembrane receptor tyrosine kinases to initiate a series of signaling events. Mice lacking the encoded protein exhibit severe defects in the peripheral nervous system including a complete lack of spinal proprioceptive afferents and their peripheral sense organs. [provided by RefSeq, Sep 2016]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same isoform (a).