Nkx2-5 (NM_008700) Mouse Untagged Clone

CAT#: MC209038

Nkx2 (untagged) - Mouse NK2 transcription factor related, locus 5 (Drosophila) (Nkx2-5), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nkx2-5
• Synonyms: Csx; Nkx-2.5; Nkx2.5; tinman
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_008700, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTTCCCCAGCCCTGCGCTCACACCCACGCCTTTCTCAGTCAAAGACATCCTGAACCTGGAGCAGCAGC
  AGCGTAGCCTGGCGTCTGGGGACCTGTCTGCGCGCCTCGAGGCCACCCTGGCCCCTGCCTCCTGCATGCT
  GGCCGCCTTCAAGCCCGAGGCCTACTCTGGCCCCGAGGCGGCAGCGTCCGGCCTGGCAGAGCTGCGCGCG
  GAGATGGGCCCCGCGCCTTCGCCCCCCAAGTGCTCTCCTGCTTTCCCAGCCGCCCCCACATTTTACCCGG
  GAGCCTACGGTGACCCTGACCCAGCCAAAGACCCTCGGGCGGATAAAAAAGAGCTGTGCGCGCTGCAGAA
  GGCAGTGGAGCTGGACAAAGCCGAGACGGATGGCGCCGAGAGACCACGCGCACGGCGGCGACGGAAGCCA
  CGCGTGCTCTTCTCGCAGGCGCAGGTCTACGAGCTGGAGCGGCGCTTCAAGCAACAGCGGTACCTGTCGG
  CGCCAGAGCGCGACCAGCTGGCCAGCGTGCTGAAGCTCACGTCCACGCAGGTCAAGATCTGGTTCCAGAA
  CCGTCGCTACAAGTGCAAGCGACAGC:GGCAGGACCAGACTCTGGAGCTTCTGGGGCCGCCGCCGCCGCC
  CGCGCGCAGGATCGCGGTGCCCGTGCTGGTGCGCGACGGGAAGCCCTGCCTGGGGGACCCCGCGGCCTAC
  GCTCCCGCCTACGGCGTGGGTCTCAATGCCTATGGCTACAACGCCTACCCCTACCCCAGCTACGGCGGCG
  CGGCCTGCAGTCCCGGCTACAGCTGCGCCGCCTACCCCGCTGCGCCCCCCGCCGCGCAGCCCCCCGCCGC
  CTCCGCCAACAGCAACTTCGTGAACTTTGGCGTCGGGGACTTGAACACCGTGCAGAGTCCCGGGATGCCG
  CAGGGCAATTCGGGCGTCTCCACGCTGCACGGCATCCGAGCCTGGTAG
  
  
• Restriction Sites: SgfI-RsrII
• ACCN: NM_008700
• Insert Size: 957 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC139299, AAI39300
• RefSeq Size: 1110 bp
• RefSeq ORF: 957 bp
• Locus ID: 18091
• UniProt ID: P42582
• Cytogenetics: 17 13.6 cM
• Gene Summary: Implicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4. Binds to the core DNA motif of NPPA promoter. It is transcriptionally controlled by PBX1 and acts as a transcriptional repressor of CDKN2B. Together with PBX1, it is required for spleen development through a mechanism that involves CDKN2B repression.[UniProtKB/Swiss-Prot Function]