Nkx2-2 (NM_010919) Mouse Untagged Clone

CAT#: MC209037

Nkx2 (untagged) - Mouse NK2 transcription factor related, locus 2 (Drosophila) (Nkx2-2), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nkx2-2
• Synonyms: Nkx-2.2; Nkx2.2; tinman
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209037 representing NM_010919
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGCTGACCAACACAAAGACGGGGTTTTCAGTCAAGGACATCTTGGACCTTCCGGACACCAACGATG
  AAGACGGCTCGGTGGCCGAAGGGCCAGAGGAGGAGAGCGAAGGGCCGGAGCCCGCCAAGAGGGCCGGGCC
  GCTGGGGCAGGGCGCCCTGGACGCTGTGCAGAGCCTGCCCCTTAAGAGCCCTTTCTACGACAGCAGCGAC
  AACCCCTACACTCGCTGGCTGGCCAGCACCGAGGGCCTCCAATACTCCCTGCACGGGCTGGCGGCCAGCG
  CTCCCCCCCAAGACTCGAGCTCCAAATCCCCAGAGCCCTCGGCTGACGAGTCACCGGACAATGACAAGGA
  GACCCAGGGCGGCGGGGGGGACGCAGGCAAGAAGCGGAAGCGCCGAGTGCTCTTCTCCAAAGCGCAGACC
  TACGAGCTGGAGCGGCGCTTCCGGCAGCAGCGGTACCTGTCGGCGCCCGAGCGCGAGCACCTGGCCAGCC
  TCATCCGTCTCACGCCGACACAGGTCAAGATCTGGTTCCAGAACCATCGCTACAAGATGAAACGTGCCCG
  GGCGGAGAAAGGTATGGAGGTGACGCCTCTGCCCTCGCCGCGCCGTGTGGCAGTGCCGGTCTTGGTCAGG
  GACGGCAAACCGTGCCACGCGCTCAAAGCCCAGGACCTGGCAGCCGCCACCTTCCAGGCAGGCATCCCCT
  TTTCCGCCTACAGCGCGCAGTCGCTGCAGCACATGCAGTACAACGCCCAGTACAGCTCGGCCAGCACCCC
  CCAGTACCCGACAGCACACCCCCTGGTCCAGGCCCAGCAGTGGACTTGGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_010919
• Insert Size: 822 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010919.2, NP_035049.1
• RefSeq Size: 2049 bp
• RefSeq ORF: 822 bp
• Locus ID: 18088
• UniProt ID: P42586
• Cytogenetics: 2 72.63 cM
• Gene Summary: Transcriptional activator involved in the development of insulin-producting beta cells in the endocrine pancreas (PubMed:11076772). May also be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance. Binds to elements within the NEUROD1 promoter (PubMed:19759004).[UniProtKB/Swiss-Prot Function]