Myf5 (NM_008656) Mouse Untagged Clone

CAT#: MC209001

Myf5 (untagged) - Mouse myogenic factor 5 (Myf5), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Myf5
• Synonyms: B130010J22Rik; bHLHc2; Myf-5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209001 representing NM_008656
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACATGACGGACGGCTGCCAGTTCTCCCCTTCTGAGTACTTCTATGAAGGCTCCTGTATCCCCTCAC
  CAGAGGATGAGTTTGGGGACCAGTTTGAGCCAAGAGTAGCAGCCTTCGGAGCACACAAAGCTGAGCTGCA
  GGGCTCAGACGATGAGGAGCACGTGCGTGCACCTACCGGCCACCACCAGGCTGGCCACTGCCTCATGTGG
  GCCTGCAAAGCTTGCAAGAGGAAGTCCACTACCATGGATCGGCGCAAGGCCGCCACCATGCGCGAGCGTA
  GACGCCTGAAGAAGGTCAACCAAGCTTTCGAGACGCTCAAGAGGTGCACCACCACCAACCCTAACCAGAG
  ACTCCCCAAGGTGGAGATCCTCAGGAATGCCATCCGCTACATTGAGAGCCTCCAGGAGCTGCTGAGGGAA
  CAGGTGGAGAACTATTACAGCCTGCCGGGACAGAGCTGCTCTGAGCCCACCAGCCCCACCTCCAACTGCT
  CTGACGGCATGCCTGAATGTAACAGCCCTGTCTGGTCCCGAAAGAACAGCAGCTTTGACAGCATCTACTG
  TCCTGATGTATCAAATGCATGTGCTGCAGATAAAAGCTCCGTGTCCAGCTTGGATTGCTTGTCCAGCATT
  GTGGATCGGATCACGTCTACAGAGCCATCCGAGCTGGCTCTTCAGGACACAGCTTCCCTCTCTCCAGCGA
  CCAGCGCCAACTCACAGCCTGCTACCCCGGGACCCTCCAGCTCCAGACTTATCTATCACGTATTATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008656
• Insert Size: 768 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008656.5, NP_032682.1
• RefSeq Size: 2083 bp
• RefSeq ORF: 768 bp
• Locus ID: 17877
• UniProt ID: P24699
• Cytogenetics: 10 55.95 cM
• Gene Summary: Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYOG and MYOD1, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.[UniProtKB/Swiss-Prot Function]