Mxi1 (NM_001008542) Mouse Untagged Clone

CAT#: MC208997

Mxi1 (untagged) - Mouse Max interacting protein 1 (Mxi1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mxi1
• Synonyms: bHLHc11; Gm10197; Mad2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC208997 representing NM_001008542
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAAACGCGGGCGGCCGCGCAAGGAGGCGCGCTGCGAGGGCGCGGGGCTGGTCCCCGTCGCGCCCC
  CGGCCATGCCCCCGGCCGCGGCCGCGCCCCAGCCCCCGGCGCAGCCGGAGGAGCCGGCGGGGGCCAAGCC
  CCGGTGCCCCTTCTCGGACATTTTCAACACCAGCGAGAACTCGATGGAGAAGCACATCAACACTTTTCTG
  CAGAACGTGCAGATTCTGCTCGAGGCAGCCAGCTACCTGGAGCAGATCGAGAAAGAAAACAAAAAGTGTG
  AACATGGCTACGCCTCATCGTTCCCCTCCATGCCGAGCCCCCGGCTACAGCACTCGAAGCCCCCACGGAG
  GTTGAGCCGGGCACAGAAACACAGCAGTGGAAGCAGCAACACCAGCACTGCCAACAGATCTACACACAAT
  GAGTTGGAAAAGAACCGACGAGCTCACCTGCGCCTGTGTTTAGAACGCTTGAAAGTTCTGATCCCGCTGG
  GCCCAGACTGCACCAGGCACACAACACTCGGTTTGCTCAACAAAGCCAAAGCACACATCAAGAAACTTGA
  AGAAGCGGAGAGGAAGAGCCAGCACCAGCTAGAGAACTTGGAACGAGAACAGAGGTTTTTAAAGCGGCGA
  CTGGAACAGCTGCAGGGGCCTCAGGAGATGGAGCGGATACGAATGGACAGCATTGGATCAACCATCTCTT
  CAGATCGCTCGGATTCAGAGCGAGAGGAGATTGAAGTGGATGTGGAAAGCACAGAGTTCTCCCATGGAGA
  AGCAGACAGTGTCAGTACCACCAGCATCAGTGACCTTGACGACCACAGCAGCCTGCAGAGTGTCGGGAGT
  GACGAGGGTTATTCCAGTGCCAGTGTCAAACTCTCCTTCGCGTCCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001008542
• Insert Size: 888 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001008542.2, NP_001008542.2
• RefSeq Size: 5156 bp
• RefSeq ORF: 888 bp
• Locus ID: 17859
• Cytogenetics: 19 47.53 cM
• Gene Summary: This gene encodes a protein containing a helix-loop-helix domain characteristic of transcription factors, which allows heterodimerization and sequence-specific DNA binding. The encoded protein is related to a family of Myc/Max/Mad proteins that are involved in the regulation of several cellular processes. The protein encoded by this gene is a transcriptional repressor thought to negatively regulate Myc function. Three alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2), also referred to as SRalpha, differs in the 5' UTR and 5' coding region, compared to variant 1. The resulting isoform (b) is longer and has a distinct N-terminus compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.