Lmx1b (NM_010725) Mouse Untagged Clone

CAT#: MC208897

Lmx1b (untagged) - Mouse LIM homeobox transcription factor 1 beta (Lmx1b), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Lmx1b
• Synonyms: Icst; LMX1.1; LMX1.2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC208897 representing NM_010725
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGGACGGCATCAAGATGGAGGAGCACGCCCTTCGCCCCGGGCCCGCCACCCTGGGGGTGCTGCTGG
  GCTCCGACTGCCCGCATCCCGCCGTCTGCGAGGGCTGCCAGCGGCCCATCTCCGACCGCTTCCTGATGCG
  AGTCAACGAGTCGTCCTGGCACGAGGAGTGTTTGCAGTGCGCGGCATGTCAGCAAGCCCTCACCACCAGC
  TGCTACTTCCGGGATCGGAAACTGTACTGCAAACAAGACTACCAACAGCTCTTCGCGGCAAAGTGCAGCG
  GCTGCATGGAGAAGATCGCCCCTACCGAGTTCGTCATGCGGGCGCTGGAGTGTGTGTACCACTTGGGCTG
  TTTCTGCTGCTGTGTGTGCGAGAGGCAACTGCGCAAGGGGGACGAGTTCGTGCTCAAGGAGGGCCAGCTG
  CTGTGCAAGGGTGACTATGAGAAGGAGAAAGACCTGCTCAGCTCCGTGAGCCCGGACGAGTCTGACTCTG
  TGAAGAGTGAGGATGAAGATGGAGACATGAAGCCGGCCAAGGGGCAGGGCAGCCAGAGTAAAGGCAGTGG
  AGATGACGGGAAAGACCCGAGAAGGCCCAAACGGCCCCGAACCATCCTCACCACACAGCAGCGAAGAGCT
  TTCAAGGCATCCTTTGAGGTCTCCTCCAAGCCCTGTCGGAAGGTCCGAGAGACATTGGCAGCAGAGACAG
  GCCTCAGCGTGCGTGTGGTCCAGGTCTGGTTTCAGAACCAAAGAGCAAAGATGAAGAAGCTGGCCCGGAG
  ACACCAGCAACAGCAGGAGCAGCAGAACTCCCAGCGGCTGGGCCAAGAGGTTCTGTCAAGCCGCATGGAG
  GGCATGATGGCCTCCTACACGCCGCTGGCCCCTCCGCAGCAGCAGATCGTGGCCATGGAGCAGAGCCCCT
  ACGGAAGTAGCGACCCCTTCCAACAGGGCCTCACGCCGCCCCAAATGCCAGGGAACGACTCCATCTTCCA
  CGATATCGATAGTGATACCTCCCTCACCAGCCTCAGCGACTGCTTCCTCGGCTCTTCCGACGTGGGCTCC
  CTGCAGGCCCGCGTGGGGAACCCCATTGACCGGCTCTACTCCATGCAGAGCTCCTACTTTGCCTCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_010725
• Insert Size: 1119 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010725.2, NP_034855.2
• RefSeq Size: 1219 bp
• RefSeq ORF: 1119 bp
• Locus ID: 16917
• UniProt ID: O88609
• Cytogenetics: 2 22.48 cM
• Gene Summary: Essential for the specification of dorsal limb fate at both the zeugopodal and autopodal levels.[UniProtKB/Swiss-Prot Function]