Kcnk4 (NM_008431) Mouse Untagged Clone

CAT#: MC208813

Kcnk4 (untagged) - Mouse potassium channel, subfamily K, member 4 (Kcnk4), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kcnk4
• Synonyms: MLZ-622; Tex40; TRAAK; TRAAKt
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC208813 representing NM_008431
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCAGCACCACACTCCTGGCTCTGCTGGCACTGGTGCTGCTTTACTTGGTATCTGGGGCTCTAGTGT
  TCCAGGCTCTGGAGCAGCCTCACGAGCAGCAGGCTCAGAAGAAAATGGATCATGGCCGAGACCAGTTTCT
  GAGGGACCATCCCTGTGTGAGCCAGAAGAGCCTGGAGGATTTCATCAAGCTCCTGGTTGAAGCCCTGGGA
  GGGGGCGCAAACCCAGAAACCAGCTGGACCAATAGCAGCAACCACTCATCAGCTTGGAACCTGGGCAGCG
  CCTTCTTTTTCTCGGGGACCATCATCACTACCATCGGCTATGGCAATATAGTCTTACACACAGATGCCGG
  GCGTCTCTTTTGTATCTTCTATGCACTGGTGGGGATCCCACTGTTCGGGATGCTGCTGGCGGGAGTCGGG
  GACCGGCTGGGCTCCTCTCTGCGCCGGGGCATCGGCCACATCGAAGCAATCTTCTTGAAGTGGCATGTGC
  CACCGGGGCTGGTGAGAAGTCTGTCCGCAGTGCTCTTCCTGCTGATCGGCTGCCTGCTCTTTGTCCTCAC
  TCCTACCTTCGTGTTCTCCTACATGGAGAGCTGGAGCAAGTTAGAAGCCATCTACTTTGTTATAGTGACT
  CTCACCACTGTAGGCTTTGGCGATTATGTACCCGGCGATGGCACCGGGCAGAACTCTCCAGCCTACCAGC
  CGCTGGTGTGGTTCTGGATCTTGTTTGGCCTAGCCTACTTCGCCTCAGTGCTCACCACCATCGGCAACTG
  GTTGCGAGCAGTGTCCCGCCGAACTCGGGCAGAGATGGGTGGCCTAACGGCACAGGCTGCTAGCTGGACC
  GGCACAGTGACAGCGCGAGTGACCCAGCGAACTGGGCCCAGCGCCCCGCCGCCAGAGAAGGAGCAACCAC
  TCCTGCCCTCCTCTTTGCCGGCACCGCCTGCTGTTGTTGAGCCAGCCGGCAGGCCCGGCTCCCCTGCACC
  CGCAGAGAAGGTTGAGACTCCGTCCCCGCCCACGGCCTCAGCTCTGGATTACCCCAGTGAGAATCTGGCC
  TTCATCGACGAGTCCTCAGACACGCAGAGTGAGCGTGGCTGTGCCCTGCCTCGGGCTCCTCGGGGTCGCC
  GCCGACCCAACCCATCCAAAAAGCCTTCCAGACCCCGGGGTCCTGGGCGACTCCGAGACAAGGCCGTGCC
  GGTGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008431
• Insert Size: 1197 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008431.2, NP_032457.1
• RefSeq Size: 1757 bp
• RefSeq ORF: 1197 bp
• Locus ID: 16528
• UniProt ID: O88454
• Cytogenetics: 19 5.08 cM
• Gene Summary: Voltage-insensitive potassium channel (PubMed:9628867). Channel opening is triggered by mechanical forces that deform the membrane. Channel opening is triggered by raising the intracellular pH to basic levels (By similarity). The channel is inactive at 24 degrees Celsius (in vitro); raising the temperature to 37 degrees Celsius increases the frequency of channel opening, with a further increase in channel activity when the temperature is raised to 42 degrees Celsius (By similarity). Plays a role in the sensory perception of pain caused by pressure (PubMed:19279663). Plays a role in the perception of pain caused by heat (PubMed:19279663).[UniProtKB/Swiss-Prot Function]