Kcnj2 (NM_008425) Mouse Untagged Clone

CAT#: MC208804

Kcnj2 (untagged) - Mouse potassium inwardly-rectifying channel, subfamily J, member 2 (Kcnj2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kcnj2
• Synonyms: IRK1; Kcnf1; Kir2.1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC208804 representing NM_008425
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAGTGTGAGAACCAACCGCTACAGCATCGTCTCTTCGGAGGAAGATGGCATGAAGCTGGCCACTA
  TGGCAGTTGCCAATGGCTTTGGGAATGGCAAGAGTAAAGTCCATACCCGACAACAGTGCAGGAGCCGCTT
  TGTGAAGAAAGATGGTCATTGCAATGTTCAGTTTATCAACGTGGGTGAGAAGGGACAGAGGTACCTGGCA
  GACATCTTTACTACCTGTGTCGACATCCGCTGGAGGTGGATGCTGGTTATCTTCTGTCTTGCCTTCGTGC
  TCTCCTGGCTGTTCTTTGGCTGTGTGTTTTGGTTGATAGCCCTGCTCCATGGGGATCTAGATACTTCTAA
  AGTGAGCAAAGCATGCGTGTCAGAGGTCAACAGCTTCACGGCTGCCTTCCTCTTCTCCATCGAGACCCAG
  ACAACCATTGGCTATGGTTTCAGGTGTGTGACAGACGAGTGCCCAATTGCTGTCTTCATGGTGGTATTCC
  AGTCAATCGTAGGCTGCATCATTGACGCCTTCATCATTGGTGCAGTCATGGCGAAGATGGCAAAGCCAAA
  GAAGAGAAATGAGACTCTTGTCTTCAGTCACAATGCTGTGATTGCCATGAGGGATGGCAAACTCTGCTTG
  ATGTGGAGAGTGGGTAACCTTCGAAAGAGCCACCTTGTGGAAGCTCATGTCCGGGCACAGCTTCTCAAAT
  CTAGGATCACTTCAGAAGGGGAGTATATCCCTTTGGACCAGATAGACATCAATGTTGGTTTTGATAGTGG
  AATTGACCGCATATTTCTAGTGTCCCCCATCACTATCGTTCACGAAATAGATGAAGACAGCCCTTTATAT
  GACTTGAGTAAGCAGGACATTGACAATGCAGACTTTGAAATTGTTGTCATACTGGAAGGCATGGTGGAGG
  CGACTGCCATGACAACTCAATGCCGGAGTTCGTATCTGGCCAATGAAATTCTCTGGGGTCACCGCTATGA
  GCCAGTGCTCTTTGAAGAGAAACACTACTATAAAGTAGACTATTCAAGATTCCATAAGACTTATGAAGTA
  CCTAACACCCCCCTTTGTAGTGCCAGAGACTTAGCAGAGAAGAAATACATCCTTTCAAATGCAAATTCAT
  TTTGCTATGAAAATGAAGTTGCCCTAACAAGCAAAGAGGAAGAGGAGGATAGTGAGAACGGAGTCCCAGA
  GAGCACAAGCACAGACTCACCTCCTGGCATAGATCTCCACAACCAGGCAAGCGTACCTCTAGAGCCCAGG
  CCCTTAAGGCGAGAATCGGAGATATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008425
• Insert Size: 1287 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008425.4, NP_032451.1
• RefSeq Size: 5468 bp
• RefSeq ORF: 1287 bp
• Locus ID: 16518
• UniProt ID: P35561
• Cytogenetics: 11 75.23 cM
• Gene Summary: Probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium.[UniProtKB/Swiss-Prot Function]