Hsd3b3 (NM_001161743) Mouse Untagged Clone

CAT#: MC208718

Hsd3b3 (untagged) - Mouse hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 3 (Hsd3b3), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Hsd3b3
• Synonyms: 9030618K22Rik; AI790201
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC208718 representing NM_001161743
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTGGGTGGAGCTGCCTGGTGACTGGAGCAGGAGGGTTTTTGGGCCAGAGGATCATCCAGTTGTTGG
  TGCAGGAGAAAGATCTTGAGGAGATCAGGGTCCTGGACAAGGTCTTCAAACCTGAAACCAGGGAGCAATT
  CTTCAACCTAGGGACAAGCATCAAGGTGACAGTGTTGGAAGGAGACATTCTTGACACCCAGTACCTGAGG
  AGAGCTTGCCAGGGCATCTCTGTTGTCATCCATACTGCTGCCATCATTGATGTCACAGGTGTCATTCCCA
  GGCAGACCATCCTAGATGTCAATCTGAAAGGTACCCAGAACTTATTGGAGGCCTGTATCCAAGCCAGTGT
  GCCAGCCTTCATCTTCTCCAGCTCAGTTGACGTTGCAGGGCCCAACTCTTACAAGGACATTGTCCTGAAT
  GGCCACGAGGACGAGCATCGTGAAAGCACATGGTCTGACCCATACCCATACAGCAAAAAGATGGCTGAGA
  AGGCAGTGCTGGCAGCCAATGGGAGCATGCTGAAAAATGGTGGCACTTTGCAAACTTGTGCATTAAGGCC
  CATGTGCATTTATGGGGAGAGAAGTCAATTCCTTTCTAACACAATAATTAAGGCCCTCAAAAATAAGTTT
  ATTCTGAGAGGTGGGGGCAAATTCTCCACAGCCAACCCAGTATATGTGGGCAATGTGGCCTGGGCACACA
  TTCTGGCTGCCAGGGGCCTTCGAAACCCCAAGAAGTCACCAAATATCCAAGGAGAGTTCTACTACATCTC
  AGATGATACCCCTCACCAAAGTTATGATGATTTAAATTACACCCTGAGCAAGGAGTGGGGCTTCTGCCTC
  AATTCCAGGTGGTACCTTCCTGTGCCCATACTGTACTGGCTTGCCTTCCTGCTGGAAACTGTGAGCTTCC
  TGCTGAGTCCAATCTACAGATATATACCTCCCTTTAACCGCCACTTGGTCACACTGACAGCTAGTACGTT
  CACTTTCTCCTACAAGAAAGCTCAGCGAGATCTGGGCTATGAGCCACTTGTCAGCTGGGAGGAAGCCAAG
  CAGAAAACCTCAGAGTGGATCGGGACACTAGTGGAGCAGCACAGGGAGACACTGGACACAAAGTCTCAGT
  GA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001161743
• Insert Size: 1122 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001161743.1, NP_001155215.1
• RefSeq Size: 1716 bp
• RefSeq ORF: 1122 bp
• Locus ID: 15494
• UniProt ID: P26150
• Cytogenetics: 3 42.86 cM
• Gene Summary: 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1-4 encode the same isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.