Hsd3b3 (NM_001012306) Mouse Untagged Clone

CAT#: MC208715

Hsd3b3 (untagged) - Mouse hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 3 (Hsd3b3), transcript variant 5, (10ug)


  "NM_001012306" in other vectors (4)

Reconstitution Protocol

USD 330.00

3 Weeks*

Size
    • 10 ug

Product Images

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Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Symbol Hsd3b3
Synonyms 9030618K22Rik; AI790201
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC208715 representing NM_001012306
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCTGGGTGGAGCTGCCTGGTGACTGGAGCAGGAGGGTTTTTGGGCCAGAGGATCATCCAGTTGTTGG
TGCAGGAGAAAGATCTTGAGGAGATCAGGGTCCTGGACAAGGTCTTCAAACCTGAAACCAGGGAGCAATT
CTTCAGTACCCAGAACTTATTGGAGGCCTGTATCCAAGCCAGTGTGCCAGCCTTCATCTTCTCCAGCTCA
GTTGACGTTGCAGGGCCCAACTCTTACAAGGACATTGTCCTGAATGGCCACGAGGACGAGCATCGTGAAA
GCACATGGTCTGACCCATACCCATACAGCAAAAAGATGGCTGAGAAGGCAGTGCTGGCAGCCAATGGGAG
CATGCTGAAAAATGGTGGCACTTTGCAAACTTGTGCATTAAGGCCCATGTGCATTTATGGGGAGAGAAGT
CAATTCCTTTCTAACACAATAATTAAGGCCCTCAAAAATAAGTTTATTCTGAGAGGTGGGGGCAAATTCT
CCACAGCCAACCCAGTATATGTGGGCAATGTGGCCTGGGCACACATTCTGGCTGCCAGGGGCCTTCGAAA
CCCCAAGAAGTCACCAAATATCCAAGGAGAGTTCTACTACATCTCAGATGATACCCCTCACCAAAGTTAT
GATGATTTAAATTACACCCTGAGCAAGGAGTGGGGCTTCTGCCTCAATTCCAGGTGGTACCTTCCTGTGC
CCATACTGTACTGGCTTGCCTTCCTGCTGGAAACTGTGAGCTTCCTGCTGAGTCCAATCTACAGATATAT
ACCTCCCTTTAACCGCCACTTGGTCACACTGACAGCTAGTACGTTCACTTTCTCCTACAAGAAAGCTCAG
CGAGATCTGGGCTATGAGCCACTTGTCAGCTGGGAGGAAGCCAAGCAGAAAACCTCAGAGTGGATCGGGA
CACTAGTGGAGCAGCACAGGGAGACACTGGACACAAAGTCTCAGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001012306
Insert Size 957 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001012306.2, NP_001012306.2
RefSeq Size 1490 bp
RefSeq ORF 957 bp
Locus ID 15494
Cytogenetics 3 42.86 cM
Gene Summary 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (5) differs in the 5' UTR and lacks an in-frame internal exon, compared to variant 1. The resulting isoform (2) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.