Hey2 (NM_013904) Mouse Untagged Clone

CAT#: MC208661

Hey2 (untagged) - Mouse hairy/enhancer-of-split related with YRPW motif 2 (Hey2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Hey2
• Synonyms: bHLHb32; CHF1; Herp1; hesr2; Hrt2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC208661 representing NM_013904
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCGCCCTTGTGAGGAAACGACCTCCGAAAGCGACCTGGACGAGACCATCGACGTGGGGAGCGAGA
  ACAATTACCCTGGGCACGCTACAAGCTCAGTGATGAGGTCCAATTCACCGACAACTACCTCTCAGATTAT
  GGCAAGAAAGAAAAGGAGAGGGATCATAGAAAAAAGGCGTCGGGATCGAATAAATAACAGTTTATCTGAA
  TTGAGAAGACTAGTGCCAACAGCTTTTGAAAAACAAGGATCTGCCAAGTTAGAAAAGGCTGAAATATTGC
  AAATGACAGTGGATCATTTGAAGATGCTCCAGGCTACAGGGGGTAAAGGCTACTTTGATGCCCATGCTCT
  TGCCACAGACTTCATGAGCATTGGATTCCGAGAGTGCTTGACAGAAGTGGCTAGGTACCTAAGCTCAGTG
  GAAGGCCTTGACCCGTCGGACCCACTACGCGTGCGCCTTGTCTCTCATCTCAGCACCTGTGCCTCCCAGC
  GGGAGGCAGCAGTGATGACATCCTCCATGGCCCACCACCATCACCCCTTGCACCCTCACCACTGGGCAGC
  TGCTTTCCACCATCTCCCCACAGCCCTGCTCCAGCCCAATGGACTCCACACATCAGAGTCAACCCCATGT
  CGCCTATCCACATCTTCAGAAGTGCCTTCTGCTCATGGCTCTGCTCTCCTCACAGCAACGTTTGCCCATG
  CAGATTCTGCTCTTCGGATGCCATCAGGGGGCACCGTTGCACCCTGCGTGCCACCTCTCTCCACCTCTCT
  TCTGTCTCTTTCGGCCACTGTGCATGCCGCAGCTGCAGCAGCCACTGCAGCTGCACACAGCTTCCCTCTG
  TCCTTCGCAGGGGCTTTTCCCATGCTCCCGTCCAATGCAGCGGCAGCAGCCGCTGTTGCTGCTGCAACAG
  CAATCAGCCCACCCTTGTCGGTATCCGCAGCCTCCAGTCCTCAGCAGACAAGCACTGGGACAAACAATAA
  ACCTTACCAACCCTGGGGGACAGAAGTTGGAGCCTTTTAA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_013904
• Insert Size: 1020 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013904.1, NP_038932.1
• RefSeq Size: 2549 bp
• RefSeq ORF: 1020 bp
• Locus ID: 15214
• UniProt ID: Q9QUS4
• Cytogenetics: 10 A4
• Gene Summary: Transcriptional repressor which functions as a downstream effector of Notch signaling in cardiovascular development. Specifically required for the Notch-induced endocardial epithelial to mesenchymal transition, which is itself criticial for cardiac valve and septum development. May be required in conjunction with HEY1 to specify arterial cell fate or identity. Promotes maintenance of neuronal precursor cells and glial versus neuronal fate specification. Binds preferentially to the canonical E box sequence 5'-CACGTG-3'. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6 and by the neuronal bHLH factors ASCL1/MASH1 and NEUROD4/MATH3.[UniProtKB/Swiss-Prot Function]