Gp1bb (NM_010327) Mouse Untagged Clone

CAT#: MC208577

Gp1bb (untagged) - Mouse glycoprotein Ib, beta polypeptide (Gp1bb), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gp1bb
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC208577 representing NM_010327
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTCCCGGCCCCGTGGGGCGCTGAGCCTGCTGCTTCTGTTGCTAGCGCTGCTCAGCCGCCCAGCCT
  CTGGCTGCCCCGCGCCATGCAGCTGCGCAGGGACGCTCGTGGACTGTGGGCGCCGCGGGCTGACCTGGGC
  GTCGCTGCCGGCTGCCTTCCCTCCCGACACCACCGAATTGGTGCTGACCGGCAACAACCTGACGGCGCTG
  CCGCCCGGGCTTCTGGACGCGTTGCCTGCTCTGCGCGCAGCACACCTGGGCGCCAACCCCTGGCGCTGCG
  ACTGCCGCCTATTGCCGCTGCGCGCCTGGCTGGCCGGCCGCCCGGAGCGCGCGCCCTACCGCGACCTGCG
  CTGTGTCGCGCCCCCAGCGCTGCGCGGCCGCTTGCTGCCCTACGTGGCGGAGGATGAGCTGCGGGCCGCG
  TGCGCACCCGGTCTACTCTGCTGGGGAGCACTGGTGGCCCAGCTAGCACTGCTGGTCCTCGGGCTGCTAC
  ACGCGCTGCTATTGGCACTTCTACTAGGTCGCCTGCGGAGGCTGCGCGCGCGTGCGCGCGCACGCTCCAT
  CCAAGAGTTCTCTCTCACAGCCCCGCTGGTGGCCGAGTCGGCCAGAGGTGGTGCATCCTAA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_010327
• Insert Size: 621 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010327.2, NP_034457.1
• RefSeq Size: 1847 bp
• RefSeq ORF: 621 bp
• Locus ID: 14724
• UniProt ID: P56400
• Cytogenetics: 16 11.53 cM
• Gene Summary: Gp-Ib, a surface membrane protein of platelets, participates in the formation of platelet plugs by binding to von Willebrand factor, which is already bound to the subendothelium.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an internal segment, as compared to variant 1. It encodes isoform 2 which has an alternate N-terminus, as compared to isoform 1. Sequence Note: This RefSeq record was created using sequences from two different strains, C57BL/6J and C57BL/6, because no single transcript from the same strain was available for the full length of the gene.