Gna12 (NM_010302) Mouse Untagged Clone

CAT#: MC208562

Gna12 (untagged) - Mouse guanine nucleotide binding protein, alpha 12 (Gna12), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gna12
• Synonyms: AI414047; AI504261; Galpha12
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC208562 representing NM_010302
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGGGGTGGTGCGGACCCTTAGCCGCTGCTTGCTGCCGGCCGAGGCCGGAGCCCGCGAGCGCAGGG
  CGGGCGCGGCGCGCGACGCGGAGCGCGAGGCCCGACGGCGCAGCCGCGACATCGACGCGCTGCTGGCCCG
  CGAGCGGCGCGCGGTGCGGCGGCTGGTCAAGATCCTGCTGCTGGGCGCCGGCGAGAGCGGCAAGTCCACC
  TTCCTCAAGCAGATGCGCATCATCCACGGCCGGGAGTTCGACCAGAAGGCGCTGCTGGAGTTCCGCGACA
  CCATCTTCGACAACATCCTTAAGGGTTCGAGGGTTCTTGTGGACGCTCGAGACAAGCTCGGCATTCCCTG
  GCAGCACTCTGAGAACGAGAAGCACGGGATGTTTCTGATGGCCTTCGAGAACAAGGCAGGGCTGCCTGTG
  GAGCCTGCCACCTTCCAGCTCTACGTGCCAGCCCTGAGTGCCCTCTGGAGAGACTCGGGGATCAGGGAAG
  CCTTCAGCCGCAGAAGCGAGTTCCAGCTGGGTGAATCAGTGAAGTACTTCCTGGATAACTTGGACCGGAT
  TGGCCAGCTGAACTACTTCCCCAGTAAGCAAGACATCCTGCTGGCTAGAAAGGCCACCAAGGGAATCGTG
  GAACATGACTTCGTTATAAAGAAAATCCCATTTAAGATGGTGGATGTGGGCGGCCAGAGGTCACAGCGCC
  AGAAGTGGTTCCAGTGCTTCGACGGCATCACATCTATCCTGTTCATGGTGTCCTCGAGCGAGTATGACCA
  GGTCCTCATGGAGGACAGGCGCACCAACCGGCTGGTGGAGTCCATGAACATCTTCGAGACCATCGTCAAC
  AACAAGCTCTTCTTCAACGTCTCCATCATCCTCTTCCTCAACAAGATGGACCTCCTGGTGGAGAAGGTGA
  AGTCTGTGAGCATTAAGAAGCACTTCCCAGATTTCAAGGGCGACCCGCACCGGCTGGAGGACGTCCAGCG
  CTACCTGGTGCAGTGCTTCGACAGGAAGCGCAGGAACCGCAGCAAGCCCCTGTTCCACCACTTCACCACC
  GCCATAGACACCGAGAACATCCGCTTCGTGTTTCATGCTGTGAAGGACACGATCCTGCAGGAGAACCTGA
  AAGACATCATGCTGCAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_010302
• Insert Size: 1140 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010302.2, NP_034432.1
• RefSeq Size: 1880 bp
• RefSeq ORF: 1140 bp
• Locus ID: 14673
• UniProt ID: P27600
• Cytogenetics: 5 79.3 cM
• Gene Summary: Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems (PubMed:19151758, PubMed:21212405, PubMed:22609986). Activates effector molecule RhoA by binding and activating RhoGEFs (ARHGEF12/LARG) (By similarity). GNA12-dependent Rho signaling subsequently regulates transcription factor AP-1 (activating protein-1) (PubMed:19151758, PubMed:21212405). GNA12-dependent Rho signaling also regulates protein phosphatese 2A activation causing dephosphorylation of its target proteins (By similarity). Promotes tumor cell invasion and metastasis by activating RhoA/ROCK signaling pathway and up-regulating proinflammatory cytokine production (By similarity). Inhibits CDH1-mediated cell adhesion in process independent from Rho activation (By similarity). Together with NAPA promotes CDH5 localization to plasma membrane (By similarity). May play a role in the control of cell migration through the TOR signaling cascade (PubMed:22609986).[UniProtKB/Swiss-Prot Function]