Gfap (NM_010277) Mouse Untagged Clone

CAT#: MC208540

Gfap (untagged) - Mouse glial fibrillary acidic protein (Gfap), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gfap
• Synonyms: AI836096
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC208540 representing NM_010277
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCGGAGACGCATCACCTCTGCGCGCCGCTCCTATGCCTCCGAGACGGTGGTCAGGGGCCTCGGTC
  CTAGTCGACAACTGGGTACCATGCCACGCTTCTCCTTGTCTCGAATGACTCCTCCACTCCCTGCCAGGGT
  GGACTTCTCCCTGGCCGGGGCGCTCAATGCTGGCTTCAAGGAGACACGGGCGAGCGAGCGTGCAGAGATG
  ATGGAGCTCAATGACCGCTTTGCTAGCTACATCGAGAAGGTCCGCTTCCTGGAACAGCAAAACAAGGCGC
  TGGCAGCTGAACTGAACCAGCTTCGAGCCAAGGAGCCCACCAAACTGGCTGATGTCTACCAGGCGGAGCT
  TCGGGAGCTGCGGCTGCGGCTGGACCAGCTTACGGCCAACAGTGCCCGGCTGGAGGTGGAGAGGGACAAC
  TTTGCACAGGACCTCGGCACCCTGAGGCAGAAGCTCCAAGATGAAACCAACCTGAGGCTGGAGGCAGAGA
  ACAACCTGGCTGCGTATAGACAGGAGGCAGATGAAGCCACCCTGGCTCGTGTGGATTTGGAGAGAAAGGT
  TGAATCGCTGGAGGAGGAGATCCAGTTCTTAAGGAAGATCTATGAGGAGGAAGTTCGAGAACTCCGGGAG
  CAGCTGGCCCAACAGCAGGTCCACGTGGAGATGGATGTGGCCAAGCCAGACCTCACAGCGGCCCTGAGAG
  AGATTCGCACTCAATACGAGGCAGTGGCCACCAGTAACATGCAAGAGACAGAGGAGTGGTATCGGTCTAA
  GTTTGCAGACCTCACAGACGCTGCGTCCCGCAACGCAGAGCTGCTCCGCCAAGCCAAGCACGAAGCTAAC
  GACTATCGCCGCCAACTGCAGGCCTTGACCTGCGATCTGGAGTCCCTGCGCGGCACGAACGAGTCCCTAG
  AGCGGCAAATGCGCGAACAGGAAGAGCGCCATGCGCGGGAGTCGGCCAGTTACCAGGAGGCACTTGCTCG
  GCTGGAGGAGGAGGGCCAAAGCCTCAAGGAGGAGATGGCCCGCCACCTGCAGGAGTACCAGGATCTACTC
  AACGTTAAGCTAGCCCTGGACATCGAGATCGCCACCTACAGGAAATTGCTGGAGGGCGAAGAAAACCGCA
  TCACCATTCCTGTACAGACTTTCTCCAACCTCCAGATCCGAGAAACCAGCCTGGACACCAAATCCGTGTC
  AGAAGGCCACCTCAAGAGGAACATCGTGGTAAAGACTGTGGAGATGCGGGATGGTGAGGTCATTAAGGAC
  TCGAAGCAGGAGCACAAGGACGTGGTGATGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_010277
• Insert Size: 1293 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010277.3, NP_034407.2
• RefSeq Size: 2733 bp
• RefSeq ORF: 1293 bp
• Locus ID: 14580
• UniProt ID: P03995
• Cytogenetics: 11 66.48 cM
• Gene Summary: GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 3' coding region and 3' UTR, compared to variant 1, which results in a protein (isoform 2) with a shorter and distinct C-terminus when compared to isoform 1.