Elk4 (NM_007923) Mouse Untagged Clone

CAT#: MC208433

Elk4 (untagged) - Mouse ELK4, member of ETS oncogene family (Elk4), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Elk4
• Synonyms: 2310011G17Rik; A130026I01Rik; BB162516; SAP-1; Sap1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC208433 representing NM_007923
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACAGTGCCATCACTCTGTGGCAGTTCCTTCTCCAGCTCCTGCAGGAGCCTCAGAATGAGCACATGA
  TCTGCTGGACATCTAACAATGGGGAGTTCAAGCTTCTGCAGGCGGAGGAGGTGGCTCGTCTTTGGGGGAT
  TCGCAAGAACAAGCCTAATATGAATTATGATAAACTCAGCCGAGCCCTGCGATACTACTACGTAAAGAAC
  ATCATTAAAAAAGTGAATGGTCAGAAGTTTGTGTACAAGTTTGTCTCCTATCCAGAGATTTTGAAAATGG
  ACCCACTGACGGTAGGCAGGATTGAGGGAGACTGTGAAGCTTTAAATTCCATTGAAACCAGCAGTTCCAA
  AGATGTTGAGTATGGAGGAAAGGAGAGGCCACCTCAGCCTGGAGCCAAGACCTCTAGTCGCAATGACTAC
  ATACACTCTGGTTTGTATTCTTCATTTACTCTTAACTCTCTGAACACCTCCAATAAGAAGCTTTTCAAGT
  CAATAAAGATAGAGAATCCAGCGGAAAAACTAGCAGAGAAGAAAGCCCAGGAGCCAACACCGTCCGTCAT
  CAAATTTGTGACAACGCCTGCCAAAAAGCCGCCCATCGAGCCTGTCGCTGCTGCCTTTGCAACGAGCCCT
  AGTCTTTCTCCATCTTCTGAAGAAACTATCCAAGCTTTGGAGACATTAGTCTCTCCCACACTACCTTCCC
  TGGAAACCCCAGCCTCCATATCCATTCTGGCTACCACCTTTAACCCCACTCCTCCGGTTCCATCCACACC
  CCTTCCTCTAAAGGAGCCTCCCAGGACACCTTCTCCACCACTGAGTTCTAACCCAGACATTGACACAGAC
  ATTGAGTCAGTGGCTTCTCAGCCAATGGAACTTCCAGAGAACTTATCACTGGAGCCTAAAAATGAGGATT
  CAGCTTTGCCAGAAAAGGACAAAACAAATAACTCATCAAGATCTAAGAAGCCCAAAGGGTTAGAACTGAC
  ACCCGCCCTTGTGGTCACAGGCAGTGACCCCAGCCCCTTGGGGATCCTAAGCCCATCTCTCCCGACAGCG
  TCTCTTACACCAGCACTCTTTTCACAGACTCCCATCTTGCTGACTCCGAGCCCCTTGCTCTCCAGCATCC
  ACTTTTGGAGCACTCTCAGCCCGTTTGCTCCCTTGAGCCCAGCCAGACTGCAAGGTGCTAATACGCTTTT
  CCAGTTTCCCTCTGTACTCAACAGTCATGGCCCGTTCACTCTGTCAGGCCTGGACGGACCTTCCACTCCG
  GGCCCATTTTCTCCAGACCTGCAGAAGACATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_007923
• Insert Size: 1293 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007923.2, NP_031949.2
• RefSeq Size: 3671 bp
• RefSeq ORF: 1293 bp
• Locus ID: 13714
• UniProt ID: P41158
• Cytogenetics: 1 E4
• Gene Summary: Involved in both transcriptional activation and repression. Interaction with SIRT7 leads to recruitment and stabilization of SIRT7 at promoters, followed by deacetylation of histone H3 at 'Lys-18' (H3K18Ac) and subsequent transcription repression. Forms a ternary complex with the serum response factor (SRF). Requires DNA-bound SRF for ternary complex formation and makes extensive DNA contacts to the 5'side of SRF, but does not bind DNA autonomously (By similarity).[UniProtKB/Swiss-Prot Function]