Trdmt1 (NM_010067) Mouse Untagged Clone

CAT#: MC208402

Trdmt1 (untagged) - Mouse tRNA aspartic acid methyltransferase 1 (Trdmt1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Trdmt1
• Synonyms: Dnmt2; Rnmt2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC208402 representing NM_010067
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACCTCTGCGTGTCCTGGAGCTGTACAGTGGCATTGGTGGCATGCACCACGCGCTGCGAGAAAGTC
  ATATCCCTGCACATGTGGTGGCTGCTATTGATGTGAATACTGTTGCTAATGAAGTATACAAGCATAATTT
  TCCTCATACTCACTTACTGTCAAAGACAATTGAAGGTATTTCACTGGAAGACTTTGACAAGCTATCTTTC
  AATATGATTTTAATGAGCCCTCCATGCCAGCCATTCACAAGAATTGGCCTACAGGGGGATATGACCGATC
  CAAGGACAACTAGCTTCTTGTATATTCTAGATATTCTCCCAAGATTACAAAAATTACCCAAGTATATTCT
  CTTAGAAAATGTCAAAGGTTTTGAAGTATCTTCTACAAGAGGGCTGCTGATACAAACAATAGAAGCCTGT
  GGCTTTCAGTATCAAGAGTTTCTATTGTCTCCTTCTTCTCTAGGCATTCCAAACTCGAGGCTCCGATATT
  TTCTCATTGCAAAGCTTCAGTCAGAGCCTTTCCCCTTCCAGGCCCCTGGACAGATACTGATGGAGTTTCC
  TAAAATTGTAACTGTTGAGCCACAAAAATATGCAGTAGTTGAAGAAAGTCAGCCAAGAGTGCAGAGAACT
  GGACCACGTATCTGTGCTGAGAGCAGCAGCACACAGAGTTCTGGAAAAGATACCATTCTCTTTAAGCTTG
  AGACTGTAGAAGAAAGGGACAGGAAACATCAACAAGACAGTGACCTCTCTGTGCAGATGCTGAAAGACTT
  CCTTGAAGATGGCGACACAGATGAGTACCTTTTACCACCCAAGTTATTGCTGCGATACGCTCTCTTACTA
  GATATCGTGAAGCCCACGTCCAGAAGGTCCATGTGCTTTACGAAAGGGTATGGGAGTTACATAGAGGGGA
  CAGGCTCCGTGTTACAGGCTGCAGAGGATGCGCAGATTGAGAATATCTACAAATCTCTTCCTGATTTGCC
  ACCAGAAGAAAAGATAGCTAAATTGTCAATGCTTAAACTGCGATATTTCACACCGAAAGAAATTGCAAAT
  CTCCAGGGATTTCCTCCAGAATTTGGGTTTCCTGAGAAGACAACAGTGAAACAGCGTTACCGGCTGCTTG
  GCAATAGCCTCAACGTGCATGTGGTAGCAAAACTCCTCACAGTCCTGTGTGAAGGATTTGGAAATGCCTC
  TGAGAGCTGTCACAAGATGCCGCTAATTCTAGATAGTAATTCCAAGATTCTATCTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_010067
• Insert Size: 1248 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010067.4, NP_034197.3
• RefSeq Size: 2637 bp
• RefSeq ORF: 1248 bp
• Locus ID: 13434
• UniProt ID: O55055
• Cytogenetics: 2 A1
• Gene Summary: Specifically methylates cytosine 38 in the anticodon loop of tRNA(Asp).[UniProtKB/Swiss-Prot Function]