Dmc1 (NM_010059) Mouse Untagged Clone

CAT#: MC208400

Dmc1 (untagged) - Mouse DMC1 dosage suppressor of mck1 homolog, meiosis-specific homologous recombination (yeast) (Dmc1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dmc1
• Synonyms: Dmc1h; Lim15; Mei1; Mei11; sg; sgdp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC208400 representing NM_010059
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGAGGATCAAGTTGTGCAGGAAGAATCGGGATTCCAAGATGATGAGGAATCTCTGTTTCAAGACA
  TTGACCTGTTGCAGAAGCATGGGATTAACATGGCTGATATTAAGAAACTGAAGTCTGTAGGAATCTGTAC
  CATTAAAGGGATACAAATGACAACAAGAAGAGCCCTCTGCAATGTCAAAGGCCTCTCAGAAGCCAAAGTG
  GAAAAGATTAAAGAGGCAGCCAACAAGCTTATTGAACCAGGATTCCTAACTGCATTTCAGTACAGTGAAA
  GGAGGAAGATGGTATTCCATATCACTACTGGGAGCCAGGAATTTGATAAATTACTAGGAGGTGGCATTGA
  AAGTATGGCAATTACAGAAGCTTTTGGAGAATTTCGTACTGGAAAAACTCAGCTGTCTCATACCCTCTGT
  GTGACAGCTCAACTTCCAGGAACAGGCGGCTACTCAGGTGGAAAGATTATCTTCATCGATACAGAAAATA
  CTTTCCGTCCAGATCGCCTTCGGGACATTGCTGACCGCTTCAACGTAGACCATGAAGCAGTACTGGACAA
  CGTGCTCTATGCACGTGCCTATACTAGTGAGCATCAGATGGAGCTACTTGATTATGTCGCAGCCAAGTTT
  CATGAGGAAGCTGGCATTTTCAAACTATTGATCATTGACTCAATAATGGCACTTTTTCGAGTGGATTTCA
  GTGGCCGTGGGGAATTGGCTGAACGCCAGCAAAAATTGGCCCAGATGTTGTCACGACTCCAAAAAATCTC
  AGAAGAATATAATGTTGCTGTTTTTGTGACCAATCAAATGACTGCAGATCCAGGAGCAACTATGACCTTT
  CAGGCTGATCCCAAAAAACCCATTGGTGGACACATTCTGGCTCACGCTTCCACAACAAGAATAAGCTTGC
  GCAAGGGAAGAGGAGAACTGAGGATCGCCAAGATTTATGACAGCCCCGAGATGCCTGAAAATGAAGCCAC
  CTTCGCAATAACCGCTGGAGGGATCGGGGATGCCAAGGAGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_010059
• Insert Size: 1023 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010059.3, NP_034189.1
• RefSeq Size: 2227 bp
• RefSeq ORF: 1023 bp
• Locus ID: 13404
• UniProt ID: Q61880
• Cytogenetics: 15 37.79 cM
• Gene Summary: This gene encodes a member of the superfamily of recombinases (also called DNA strand-exchange proteins). Recombinases are important for repairing double-strand DNA breaks during mitosis and meiosis. This protein, which is evolutionarily conserved, is reported to be essential for meiotic homologous recombination and may thus play an important role in generating diversity of genetic information. In mouse, deficiency of this gene causes infertility. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1).