Dlk1 (NM_001190703) Mouse Untagged Clone

CAT#: MC208392

Dlk1 (untagged) - Mouse delta-like 1 homolog (Drosophila) (Dlk1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dlk1
• Synonyms: AW742678; DLK-1; DlkI; FA1; Ly107; Peg9; pG2; pref-1; SCP1; ZOG
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC208392 representing NM_001190703
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCGCGACCGGAGCCCTCCTGCGCGTCCTCTTGCTCCTGCTGGCTTTCGGCCACAGCACCTATGGGG
  CTGAATGCGACCCACCCTGTGACCCCCAGTATGGATTCTGCGAGGCTGACAATGTCTGCAGGTGCCATGT
  TGGCTGGGAGGGTCCCCTCTGTGACAAGTGTGTAACTGCCCCTGGCTGTGTCAATGGAGTCTGCAAGGAA
  CCATGGCAGTGCATCTGCAAGGATGGCTGGGACGGGAAATTCTGCGAAATAGACGTTCGGGCTTGCACCT
  CAACCCCCTGCGCCAACAATGGAACTTGCGTGGACCTGGAGAAAGGCCAGTACGAATGCTCCTGCACACC
  TGGGTTCTCTGGAAAGGACTGCCAGCACAAGGCTGGGCCCTGCGTGATCAATGGTTCTCCCTGCCAGCAC
  GGAGGCGCCTGCGTGGATGATGAGGGCCAGGCCTCGCATGCTTCCTGCCTGTGCCCCCCTGGCTTCTCAG
  GCAACTTCTGTGAGATCGTAGCCGCAACCAACAGCTGTACCCCTAACCCATGCGAGAACGATGGCGTCTG
  CACCGACATCGGGGGTGACTTCCGTTGCCGCTGCCCAGCTGGATTCGTCGACAAGACCTGCAGCCGCCCG
  GCCATCTGCTTCACCATCCTGGGCGTGCTCACCAGCCTGGTGGTGCTGGGCACCGTGGCCATCGTCTTTC
  TCAACAAGTGCGAAACCTGGGTGTCCAACCTGCGCTACAACCACATGCTTCGCAAGAAGAAGAACCTCCT
  GTTGCAGTATAACAGCGGCGAGGAGCTGGCGGTCAATATCATCTTCCCCGAGAAGATTGACATGACCACT
  TTCAACAAGGAGGCTGGTGATGAGGAGATCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001190703
• Insert Size: 873 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001190703.1, NP_001177632.1
• RefSeq Size: 4054 bp
• RefSeq ORF: 873 bp
• Locus ID: 13386
• Cytogenetics: 12 60.17 cM
• Gene Summary: May have a role in neuroendocrine differentiation. Inhibits adipocyte differentiation.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.