Dio2 (NM_010050) Mouse Untagged Clone

CAT#: MC208391

Dio2 (untagged) - Mouse deiodinase, iodothyronine, type II (Dio2), (10ug), (Note: selenocysteine protein, Internal stop codon present. see reference data summary below)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Symbol: Dio2
• Synonyms: 5DII; AI324267; DIOII
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_010050, the custom clone sequence may differ by one or more nucleotides
  
  
  TGACTGGGGAAGCAGAGTGCCCAGGAGACTGACCGAGGAGGCAGAGAAGATGGGACTCCTCAGCGTAGAC
  TTGCTGATCACCCTGCAGATCCTGCCAGTCTTTTTCTCCAACTGCCTCTTCCTGGCGCTCTATGACTCGG
  TCATTCTGCTCAAGCACGTGGCGTTGCTTCTGAGCCGCTCCAAGTCCACTCGCGGAGAGTGGAGGCGCAT
  GCTGACCTCAGAAGGGCTGCGCTGTGTCTGGAACAGCTTCCTCCTAGATGCCTACAAACAGGTTAAACTG
  GGTGAAGATGCTCCCAATTCCAGTGTGGTGCACGTCTCCAATCCTGAATCAGGTAACAATTATGCCTCGG
  AGAAGACCGCTGATGGGGCCGAATGCCACCTTCTTGACTTTGCCAGTGCAGAGCGCCCACTGGTGGTCAA
  CTTTGGTTCAGCCACCTGACCACCTTTCACTAGGCAACTGCCAGCCTTCCGCCAGCTGGTGGAAGAGTTC
  TCCTCGGTGGCTGACTTCCTGTTGGTATACATTGATGAGGCTCACCCTTCGGATGGCTGGGCAGTGCCTG
  GGGACTCCTCTCTGTCTTTTGAGGTTAAGAAGCACCGGAACCAAGAGGACCGATGTGCAGCAGCTCACCA
  GCTCCTGGAGCGTTTCTCCTTGCCGCCCCAGTGTCAAGTTGTGGCTGACCGCATGGACAATAATGCCAAC
  GTAGCTTACGGGGTAGCCTTTGAACGTGTGTGCATCGTGCAGAGACGGAAAATTGCTTACTTAGGAGGGA
  AGGGCCCCTTCAGCTATAACCTACAAGAAGTCCGAAGTTGGCTGGAGAAGAATTTCAGCAAGAGATGAAT
  TCTAGATTAGCTGGATACAAGTGTGATTGTAATAGAGTTTATTATTTTAAAGAAATATGTAAAAGAAGTT
  GAGAACTGAACTGAATCTATTATTTCAACTGAATCCCATTGCCTCACCGAAAGACGGGGACACACCTGTC
  AGAAGACCTTGAACCTCTTAACATCTCAATACTTTCTCTAGTACACAAATGACATCTGGCTAAATAGCAG
  CCCTTTTACCTCCCTTCTGAGCGAATTGATCCAAACTGGAGTGCAAGAAACCCTGTTGCAGCCTGTATTC
  ATTCTTGCTTGCGAAAGAAGTGATTCTGTCCTGCGTGTGCTCTGGGACCAGAGGACCATAAGTTCTCAGA
  ATACTTATTGTTAAGGGAGCTACTACCTATGATCTGATTAAGTGTATTGTCTCAGCTTTAGTCACCAGAA
  ACAAACCCAACTGGAAAGTCCCCCAGATACATACTTACGATGTGATCTTGAAGCCATGCCATGCAGTTAG
  GACTGAAGTGTTAGTTCCAGCCATGCAATGTCCCGGTTAGTCTCTGTAGCCCCGAATTCCCAAGTGTGAC
  AAGCAAAGACAGAGGAAGCAGAAAGATGAGGAAATCGCATAGTGTTTTTACCTGGAAGCTGTAGGTAATG
  AGTGTTGTTCAACAGCATCAGCATTGAGGCATGTACACAGCTGGGAAAACAAAGGCGATAACTAAAAGTA
  GCACGGGCAGGATGCACACGGAAAGGAGATGTGTAGCAATATTCCTACACTGAAAGGAAGTGGGCGATGG
  CAAAGATAGGTGAACGTTTTTAATGTGGAGAGAAGTACTTTATGGGACTAATTGAGATATACCCATTTCA
  GTGGATAATATCCGTTCAGAGAAAACTCCAGGGGGTCTGGTGCTGATGTGTTGTTCCTGCCAAATTCCTA
  CAGTCACAATGCTACACCCAGCTCCATTCCTAACCTCTGTAGCATTGTTTTCTTTGGGTGCTGAGTGAAG
  GGTGTAAGAGGCAGGTACACTAACCATTCAGTTTCTGCCTTTCTCCTCCAGTAAAAGAACTGTAATGATA
  AGGGATTTCTTCACATAAACATGAGAGCAATTTATTCTGAGAATCTCTACCTATAGTTTTGAGGGCTTAC
  ACAAGCTTATTCTTGGTACCACAGGAATTTGGTTAAATGGAATTTCCTTTGACATTTGAATTTGGACTTG
  GTTCTTTTTTGTTCAACTGGGATTAACCCATTAGTTGACACTTAATAGCTGTGTACATACCTATGATAAA
  AACTAGATATGAATACATATCTCTTAAAATACAAGAGACACAGCTGTACCTACAAGCAATACCTAAAGAT
  CACAACATAAAAAACACTCCACAAAAAACCCAAAACCAAAACACACCAACAGATCAACCAATGTATTGTA
  ATTATTTTTTAAACTCAAAGACATTAAGATCTCAACATATGGTTATAGTTACATGCCAAATCAAGGGTCT
  TATGTCTATTCCATTCAATAATTAAACCATAATATTGAGTTATTTGGAAAAAGACAATTGAAATTTAAAA
  GTACATTGTTAAAATATATTGTCGCAAATGTGGAAAATAGGAAGTGGGTATGCATAAAACAAGAGTCTTG
  TTTTTAAAAATGGGAGCAAGAAATAAGTCAGATCGCTAACCCATATATTGTTTAGTCATGGAAGCAGCAC
  TATGGCTAATGTTTTTGGCAGCATCTAAGTTCTCCAGTGTTTTAAATTCTGTAAAAGACACACCCACAAG
  GAAACACAGTGAATTCCCAAAATGCAACAGTGTCTAATGAACTTCTGTAGAATCATATTGAAGATCGATT
  ACTTCAGACTCTTCAGATGTCAAGGCCTTCACTTGAGGCTTATGAGCGATCCCTCACTCATCTTCATCTT
  CAAAATGCTGTTGCGATTGATGTGGCTCCCTAAAGAAGTAAAATGGAAAATTGGCTGCCCCACACCCACA
  CCGTCGTCCGCAAATGACCCCTTTGGTTTCCACGTGTGATAGCGAAGCAGAAAGTCAGAGAGTGGGTGGG
  GGGATGAGTTTTGGCTAACGCCATGCTAGTTGTCTGGG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_010050
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). The expression of this clone is not guaranteed due to the nature of selenoproteins.
• OTI Annotation: This clone encodes a selenoprotein containing the rare amino acid selenocysteine (Sec). Sec is encoded by UGA codon, which normally signals translational termination. Expression of this clone is not guaranteed due to the nature of selenoproteins.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC125383, AAI25384
• RefSeq Size: 2908 bp
• RefSeq ORF: 789 bp
• Locus ID: 13371
• UniProt ID: Q9Z1Y9
• Cytogenetics: 12 D3
• Gene Summary: The protein encoded by this gene belongs to the iodothyronine deiodinase family. It catalyzes the conversion of prohormone thyroxine (3,5,3',5'-tetraiodothyronine, T4) to the bioactive thyroid hormone (3,5,3'-triiodothyronine, T3) by outer ring 5'-deiodination. This gene is highly expressed in brain, placenta and mammary gland. It is thought to be responsible for the 'local' production of T3, and thus important in influencing thyroid hormone action in these tissues. Knockout studies in mice suggest that this gene may play an important role in brown adipose tissue lipogenesis, auditory function, and bone formation. This protein is a selenoprotein containing the non-standard amino acid, selenocysteine (Sec), which is encoded by the UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Unlike the other two members (DIO1 and DIO3) of this enzyme family, the mRNA for this gene contains an additional in-frame UGA codon that has been reported (in human) to function either as a Sec or a stop codon, resulting in two potential isoforms with one or two Sec residues; however, only the upstream Sec (conserved with the single Sec residue found at the active site in DIO1 and DIO3) was shown to be essential for enzyme activity (PMID:10403186). In addition, the lack of conservation of the protein extension past the second TGA codon suggests that the one-Sec containing isoform represents the canonical form. [provided by RefSeq, Oct 2018]