Dcn (NM_001190451) Mouse Untagged Clone

CAT#: MC208369

Dcn (untagged) - Mouse decorin (Dcn), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dcn
• Synonyms: DC; DSPG2; PG40; PGII; PGS2; SL; SLRR1B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC208369 representing NM_001190451
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGCAACTCTCATCTTCTTCCTTCTGGCACAAGTCTCTTGGGCTGGACCATTTGAACAGAGAGGCT
  TATTTGACTTCATGCTAGAAGATGAGGCTTCTGGCATAATCCCTTATGACCCTGACAATCCCCTGATATC
  TATGTGCCCCTACCGATGCCAGTGTCATCTTCGAGTGGTGCAGTGTTCTGATCTGGGTTTGGACAAAGTG
  CCCTGGGATTTTCCACCCGACACAACCTTGCTAGACCTGCAAAACAACAAAATTACAGAGATCAAAGAAG
  GGGCCTTCAAGAACCTGAAGGACTTGCATACCTTGATCCTTGTCAACAACAAGATCAGCAAAATCAGTCC
  AGAGGCATTCAAACCTCTCGTGAAGTTGGAAAGGCTTTACCTGTCTAAGAACCAACTAAAGGAACTGCCT
  GAAAAAATGCCCAGAACTCTCCAGGAACTTCGTGTCCATGAGAATGAGATCACCAAGCTGCGGAAATCCG
  ACTTCAATGGACTGAACAATGTGCTTGTCATAGAACTGGGCGGCAACCCACTGAAAAACTCTGGGATTGA
  AAACGGAGCCTTCCAGGGACTGAAGAGTCTCTCATACATTCGCATCTCAGACACCAACATAACTGCGATC
  CCTCAAGGTCTGCCTACTTCTCTCACTGAAGTGCATCTAGATGGCAACAAGATCACCAAGGTTGATGCAC
  CCAGCCTGAAAGGACTGATTAATTTGTCTAAACTGGGATTGAGCTTCAACAGCATCACCGTTATGGAGAA
  TGGCAGTCTGGCCAATGTTCCTCATCTGAGGGAACTCCACTTGGACAACAACAAACTCCTCAGGGTGCCT
  GCTGGGCTGGCACAGCATAAGTATATCCAGGTCGTCTACCTTCACAACAACAACATCTCCGCAGTTGGGC
  AAAATGACTTCTGCCGAGCTGGACACCCCTCTCGAAAGGCTTCCTACTCGGCTGTGAGTCTTTACGGCAA
  CCCTGTCCGGTATTGGGAAATCTTTCCAAACACCTTCAGATGTGTCTATGTGCGTTCTGCCATTCAACTT
  GGAAACTACAAGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001190451
• Insert Size: 1065 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC132521, AAI32522
• RefSeq Size: 1520 bp
• RefSeq ORF: 1065 bp
• Locus ID: 13179
• UniProt ID: P28654
• Cytogenetics: 10 50.27 cM
• Gene Summary: This gene encodes a member of the small leucine-rich proteoglycan (SLRP) family of proteins. The encoded preproprotein is proteolytically processed to generate a mature protein product, which is secreted into the extracellular space to regulate collagen fibril assembly. Homozygous knockout mice for this gene exhibit enhanced tumorigenesis in a liver cancer model, and defects in collagen fibrils, leading to weakened skin and tendons. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]
  Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein.