Ctsl (NM_009984) Mouse Untagged Clone

CAT#: MC208359

Ctsl (untagged) - Mouse cathepsin L (Ctsl), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ctsl
• Synonyms: 1190035F06Rik; CatL; Ctsl1; fs; ME; MEP; nkt
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC208359 representing NM_009984
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATCTTTTACTCCTTTTGGCTGTCCTCTGCTTGGGAACAGCCTTAGCTACTCCAAAATTTGATCAAA
  CCTTTAGTGCAGAGTGGCACCAGTGGAAGTCCACACACAGAAGACTGTATGGCACGAATGAGGAAGAGTG
  GAGGAGAGCGATATGGGAGAAGAACATGAGAATGATCCAGCTACACAACGGGGAATACAGCAACGGGCAG
  CACGGCTTTTCCATGGAGATGAACGCCTTCGGTGACATGACCAATGAGGAATTCAGGCAGGTGGTGAATG
  GCTACCGCCACCAGAAGCACAAGAAGGGGAGGCTTTTTCAGGAACCGCTGATGCTTAAGATCCCCAAGTC
  TGTGGACTGGAGAGAAAAGGGTTGTGTGACTCCTGTGAAGAACCAGGGCCAGTGCGGGTCTTGTTGGGCG
  TTTAGCGCATCGGGTTGCCTAGAAGGACAGATGTTCCTTAAGACCGGCAAACTGATCTCACTGAGTGAAC
  AGAACCTTGTGGACTGTTCTCACGCTCAAGGCAATCAGGGCTGTAACGGAGGCCTGATGGATTTTGCTTT
  CCAGTACATTAAGGAAAATGGAGGTCTGGACTCGGAGGAGTCTTACCCCTATGAAGCGAAGGACGGATCT
  TGTAAATACAGAGCCGAGTTCGCTGTGGCTAATGACACAGGGTTCGTGGATATCCCTCAGCAAGAGAAAG
  CCCTCATGAAGGCTGTGGCGACTGTGGGGCCTATTTCTGTTGCTATGGACGCAAGCCATCCGTCTCTCCA
  GTTCTATAGTTCAGGCATCTACTATGAACCCAACTGTAGCAGCAAGAACCTCGACCATGGGGTTCTGTTG
  GTGGGCTATGGCTATGAAGGAACAGATTCAAATAAGAATAAATATTGGCTTGTCAAGAACAGCTGGGGAA
  GTGAATGGGGTATGGAAGGCTACATCAAAATAGCCAAAGACCGGGACAACCACTGTGGACTTGCCACCGC
  GGCCAGCTATCCTGTCGTGAATTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009984
• Insert Size: 1005 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009984.4, NP_034114.1
• RefSeq Size: 3509 bp
• RefSeq ORF: 1005 bp
• Locus ID: 13039
• UniProt ID: P06797
• Cytogenetics: 13 33.26 cM
• Gene Summary: This gene encodes a member of the peptidase C1 (papain) family of cysteine proteases. The encoded preproprotein is proteolytically processed to generate multiple protein products. These products include the activation peptide and the cathepsin L1 heavy and light chains. The mature enzyme appears to be important in embryonic development through its processing of histone H3 and may play a role in disease progression in a model of kidney disease. Homozygous knockout mice for this gene exhibit hair loss, skin thickening, bone and heart defects, and enhanced susceptibility to bacterial infection. A pseudogene of this gene has been identified in the genome. [provided by RefSeq, Aug 2015]