Csf3 (NM_009971) Mouse Untagged Clone

CAT#: MC208344

Csf3 (untagged) - Mouse colony stimulating factor 3 (granulocyte) (Csf3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Csf3
• Synonyms: Csfg; G-CSF; MGI-IG
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC208344 representing NM_009971
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCAACTTTCTGCCCAGAGGCGCATGAAGCTAATGGCCCTGCAGCTGCTGCTGTGGCAAAGTGCAC
  TATGGTCAGGACGAGAGGCCGTTCCCCTGGTCACTGTCAGCGCTCTGCCACCATCCCTGCCTCTGCCCCG
  AAGCTTCCTGCTTAAGTCCCTGGAGCAAGTGAGGAAGATCCAGGCCAGCGGCTCGGTGCTGCTGGAGCAG
  TTGTGTGCCACCTACAAGCTGTGTCACCCCGAGGAGCTGGTGTTGCTGGGCCACTCTCTGGGGATCCCGA
  AGGCTTCCCTGAGTGGCTGCTCTAGCCAGGCCCTGCAGCAGACACAGTGCCTAAGCCAGCTCCACAGTGG
  GCTCTGCCTCTACCAAGGTCTCCTGCAGGCTCTATCGGGTATTTCCCCTGCCCTGGCCCCCACCTTGGAC
  TTGCTTCAGCTGGATGTTGCCAACTTTGCCACCACCATCTGGCAGCAGATGGAAAACCTAGGGGTGGCCC
  CTACTGTGCAGCCCACACAGAGCGCCATGCCAGCCTTCACTTCTGCCTTCCAGCGCCGGGCAGGAGGTGT
  CCTGGCCATTTCGTACCTGCAGGGCTTCCTGGAGACGGCTCGCCTTGCTCTGCACCACTTGGCCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009971
• Insert Size: 627 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC120759, AAI20760
• RefSeq Size: 1001 bp
• RefSeq ORF: 627 bp
• Locus ID: 12985
• UniProt ID: P09920
• Cytogenetics: 11 62.45 cM
• Gene Summary: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.[UniProtKB/Swiss-Prot Function]