Crem (NM_001110853) Mouse Untagged Clone

CAT#: MC208329

Crem (untagged) - Mouse cAMP responsive element modulator (Crem), transcript variant 9, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Crem
• Synonyms: IC; ICER; ICERI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC208329 representing NM_001110853
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTTCTGTAGCTGGATCAGGCACTGGAAGAGGCTCCCCAGCTGTGACTCTAGTACAGTTACCTTCAG
  GCCAAACTGTACAGGTCCAGGGAGTTATTCAGACACCACATCCATCGGTTATTCAATCACCACAAATACA
  AACTGTTCAGGTAGCAACAATTGCAGAGACAGATGATTCTGCAGACTCAGAAGTAATTGATTCGCATAAA
  CGTAGAGAAATTCTTTCACGAAGACCCTCATATAGAAAAATACTGAATGAACTTTCCTCTGATGTGCCTG
  GTATTCCCAAGATTGAAGAAGAAAAATCAGAGGAAGAAGGGACACCACCTAACATTGCTACCATGGCAGT
  ACCAACTAGCATATATCAGACTAGCACGGGGCAATACATTGCTATAGCTCAAGGTGGAACAATCCAGATT
  TCTAACCCAGGATCTGATGGTGTTCAGGGACTCCAGGCATTAACAATGACAAATTCAGGAGCTCCTCCGC
  CAGGTGCTACAATTGTACAGTATGCAGCACAATCAGCCGATGGTACACAGCAGTTCTTTGTCCCAGGCAG
  CCAGGTTGTTGTTCAAGATGAGGAGACTGACCTTGCCCCAAGTCACATGGCTGCTGCCACAGGTGACATG
  CCAACTTACCAGATCCGAGCTCCTACTACTGCTTTGCCACAAGGTGTGGTGATGGCTGCCTCACCAGGAA
  GCCTGCACAGTCCCCAGCAACTAGCAGAAGAAGCAACTCGCAAGCGGGAGCTGAGGCTGATGAAAAACAG
  GGAAGCTGCTAAAGAATGTCGACGTCGAAAGAAAGAGTATGTGAAGTGTCTTGAGAGTCGAGTCGCAGTG
  CTGGAAGTTCAGAACAAGAAGCTTATAGAGGAGCTTGAAACTTTGAAAGACATTTGCTCTCCCAAAACAG
  ATTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001110853
• Insert Size: 915 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001110853.2, NP_001104323.1
• RefSeq Size: 2270 bp
• RefSeq ORF: 915 bp
• Locus ID: 12916
• Cytogenetics: 18 A1
• Gene Summary: This gene encodes a basic-leucine zipper domain-containing protein that localizes to gene promoters, where it binds to the cyclic AMP response element (CRE). Different protein isoforms encoded by this gene may function as either activators or repressors of transcription. Activity of this gene is important in multiple developmental processes, including spermatogenesis. Mutation of this gene causes male infertility. Alternative splicing and promoter usage result in multiple transcript variants for this gene. [provided by RefSeq, Oct 2012]
  Transcript Variant: This variant (9) differs in the 5'UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (9) has a shorter N-terminus, compared to isoform 1.