Cd8b1 (NM_009858) Mouse Untagged Clone

CAT#: MC208268

Cd8b1 (untagged) - Mouse CD8 antigen, beta chain 1 (Cd8b1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cd8b1
• Synonyms: Cd8b; Ly-3; Ly-C; Lyt-3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC208268 representing NM_009858
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCCATGGCTCTGGCTGGTCTTCAGTATGAAGCTGGCAGCTCTCTGGAGCAGCTCTGCCCTCATTC
  AGACCCCTTCGTCCCTGCTGGTTCAAACCAACCATACGGCAAAGATGTCCTGTGAGGTTAAAAGCATCTC
  TAAGTTAACAAGCATCTACTGGCTGCGGGAGCGCCAGGACCCCAAGGACAAGTACTTTGAGTTCCTGGCC
  TCCTGGAGTTCTTCCAAAGGAGTTTTGTATGGTGAAAGTGTGGACAAGAAAAGAAATATAATTCTTGAGT
  CTTCAGACTCAAGACGGCCCTTTCTCAGTATCATGAATGTGAAGCCAGAGGACAGTGACTTCTACTTCTG
  CGCGACGGTTGGGAGCCCCAAGATGGTCTTTGGGACAGGGACGAAGCTGACTGTGGTTGATGTCCTTCCT
  ACAACTGCCCCAACCAAGAAGACTACCCTGAAGATGAAGAAGAAGAAGCAATGCCCGTTCCCCCACCCAG
  AGACCCAGAAGGGCCTGACATGCAGCCTTACCACCCTCAGCCTGCTGGTAGTCTGCATCCTGCTTCTGCT
  GGCATTCCTCGGAGTGGCCGTCTACTTTTACTGTGTGCGGAGGAGAGCCCGAATTCACTTCATGAAACAG
  TTTCACAAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009858
• Insert Size: 642 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009858.2, NP_033988.1
• RefSeq Size: 1416 bp
• RefSeq ORF: 642 bp
• Locus ID: 12526
• UniProt ID: P10300
• Cytogenetics: 6 32.14 cM
• Gene Summary: Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. A palmitoylation site in the cytoplasmic tail of CD8B chain contributes to partitioning of CD8 into the plasma membrane lipid rafts where signaling proteins are enriched. Once LCK recruited, it initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). Additionally, plays a critical role in thymic selection of CD8+ T-cells.[UniProtKB/Swiss-Prot Function]