Gdf2 (NM_019506) Mouse Untagged Clone

CAT#: MC208198

Gdf2 (untagged) - Mouse growth differentiation factor 2 (Gdf2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gdf2
• Synonyms: Bmp; Bmp9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC208198 representing NM_019506
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCCTGGGGCCTTCCGGGTGGCCCTGCTCCCGCTGTTCCTGCTGGTCTGTGTCACACAGCAGAAGC
  CGCTGCAGAACTGGGAACAAGCATCCCCTGGGGAAAATGCCCACAGCTCCCTGGGATTGTCTGGAGCTGG
  AGAGGAGGGTGTCTTTGACCTGCAGATGTTCCTGGAGAACATGAAGGTGGATTTCCTACGCAGCCTTAAC
  CTCAGCGGCATTCCCTCCCAGGACAAAACCAGAGCGGAGCCACCCCAGTACATGATCGACTTGTACAACA
  GATACACAACGGACAAATCGTCTACGCCTGCCTCCAACATCGTGCGGAGCTTCAGCGTGGAAGATGCTAT
  ATCGACAGCTGCCACGGAGGACTTCCCCTTTCAGAAGCACATCCTGATCTTCAACATCTCCATCCCGAGG
  CACGAGCAGATCACCAGGGCTGAGCTCCGACTCTATGTCTCCTGCCAAAATGATGTGGACTCCACTCATG
  GGCTGGAAGGAAGCATGGTCGTTTATGATGTTCTGGAGGACAGTGAGACTTGGGACCAGGCCACGGGGAC
  CAAGACCTTCTTGGTATCCCAGGACATTCGGGACGAAGGATGGGAGACTTTAGAAGTATCGAGTGCCGTG
  AAGCGGTGGGTCAGGGCAGACTCCACAACAAACAAAAATAAGCTCGAGGTGACAGTGCAGAGCCACAGGG
  AGAGCTGTGACACACTGGACATCAGTGTCCCTCCAGGTTCCAAAAACCTGCCCTTCTTTGTTGTCTTCTC
  CAATGACCGCAGCAATGGGACCAAGGAGACCAGACTGGAGCTGAAGGAGATGATCGGCCATGAGCAGGAG
  ACCATGCTTGTGAAGACAGCCAAAAATGCTTACCAGGTGGCAGGTGAGAGCCAAGAGGAGGAGGGTCTAG
  ATGGATACACAGCTGTGGGACCACTTTTAGCTAGAAGGAAGAGGAGCACCGGAGCCAGCAGCCACTGCCA
  GAAGACTTCTCTCAGGGTGAACTTTGAGGACATCGGCTGGGACAGCTGGATCATTGCACCCAAGGAATAT
  GACGCCTATGAGTGTAAAGGGGGTTGCTTCTTCCCATTGGCTGATGACGTGACACCCACCAAACATGCCA
  TCGTGCAGACCCTGGTGCATCTCAAGTTCCCCACAAAGGTGGGCAAAGCCTGCTGCGTTCCCACCAAACT
  GAGTCCCATCTCCATCCTCTACAAGGATGACATGGGGGTGCCAACCCTCAAGTACCACTATGAGGGGATG
  AGTGTGGCTGAGTGTGGGTGTAGGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_019506
• Insert Size: 1287 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019506.4, NP_062379.3
• RefSeq Size: 2988 bp
• RefSeq ORF: 1287 bp
• Locus ID: 12165
• UniProt ID: Q9WV56
• Cytogenetics: 14 B
• Gene Summary: This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates cartilage and bone development, angiogenesis and differentiation of cholinergic central nervous system neurons. Homozygous null mice exhibit malformations of the vasculature and skeleton. [provided by RefSeq, Jul 2016]