Ambn (NM_009664) Mouse Untagged Clone

CAT#: MC208128

Ambn (untagged) - Mouse ameloblastin (Ambn), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ambn
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC208128 representing NM_009664
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGCATCTAAGATTCCACTTTTCAAAATGAAGGGCCTGATCCTGTTCCTGTCCCTAGTGAAAATGA
  GCCTCGCCGTGCCGGCATTTCCTCAACAACCTGGGGCTCAAGGCATGGCACCTCCTGGCATGGCTAGTTT
  GAGCCTTGAGACAATGAGACAGTTGGGAAGCTTGCAGGGACTCAACGCACTTTCTCAGTATTCTAGACTT
  GGCTTTGGAAAAGCACTTAATAGTTTATGGTTGCACGGACTTCTCCCACCGCATAACTCTTTCCCATGGA
  TAGGACCAAGGGAACATGAAACCCAGCAGCCATCCTTGCAGCCTCACCAGCCAGGACTGAAACCCTTCCT
  CCAGCCCACTGCTGCAACCGGTGTCCAGGTCACACCCCAGAAGCCAGGGCCTCAGCCTCCAATGCACCCT
  GGACAGCTGCCCTTGCAGGAAGGAGAGCTGATAGCACCAGATGAGCCGCAGGTGGCACCATCCGAAAACC
  CACCAACACCTGAGGTACCAATAATGGATTTTGCTGATCCACAATTTCCAACCGTGTTCCAGATCGCCCG
  TTCAATATCTCGGGGACCAATGGCACACAACAAAGCATCCGCTTTTTACCCAGGAATGTTTTACATGTCT
  TATGGAGCAAACCAATTGAATGCTCCTGCCAGAATTGGCTTCATGAGTTCAGAAGAAATGCCTGGAGAAA
  GAGGAAGTCCCATGGCCTATGGAACTCTGTTCCCAAGATTTGGAGGCTTCAGGCAAACCCTTAGGAGACT
  GAATCAGAATTCACCCAAGGGAGGAGACTTTACTGTGGAAGTAGATTCCCCAGTATCTGTTACCAAAGGC
  CCTGAAAAAGGAGAAGGTCCAGAAGGCTCTCCACTGCAAGAGGCCAACCCAGGCAAACGGGAAAACCCCG
  CTCTCCTTTCACAAATGGCACCTGGGGCCCATGCAGGACTTCTTGCTTTCCCCAATGACCACATCCCCAG
  TATGGCAAGGGGTCCTGCAGGGCAAAGACTCCTTGGAGTCACCCCTGCAGCTGCAGACCCACTGATCACC
  CCTGAATTAGCAGAAGTTTATGAAACCTATGGTGCTGATGTTACCACACCCCTGGGTGATGGAGAAGCAA
  CCATGGATATCACCATGTCCCCAGACACTCAGCAGCCACTGCTACCTGGAAACAAAGTGCACCAGCCCCA
  GGTGCACAACGCATGGCGTTTCCAAGAGCCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009664
• Insert Size: 1224 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009664.2, NP_033794.1
• RefSeq Size: 1874 bp
• RefSeq ORF: 1224 bp
• Locus ID: 11698
• UniProt ID: O55189
• Cytogenetics: 5 43.63 cM
• Gene Summary: This gene encodes an extracellular matrix glycoprotein that is involved in the formation of dental enamel. Mice lacking the encoded protein fail to undergo normal ameloblast differentiation and develop enamel. Mice overproducing the product of this gene develop thinner and more porous enamel, with disrupted rod patterns and abnormal crystallites. Alternate splicing of this gene results in multiple transcript variants. [provided by RefSeq, Dec 2014]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.