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Trib1 (NM_144549) Mouse Untagged Clone

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SKU
MC208084
Trib1 (untagged) - Mouse tribbles homolog 1 (Drosophila) (Trib1), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $503.00
4 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Trib1
Synonyms A530090O15Rik; TRB-1; Trb1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC208084 representing NM_144549
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGGGTCGGTCCCGTGCGTTTTGCCCTGAGCGGCGCCTCGCAGCCCCGCGGTCCGGGTCTACTGTTTC
CGGCTGCCCGGGGAACCCCGGCCAAACGCCTGCTGGACACGGACGACGCGGGGGCAGTGGCGGCCAAGTG
TCCGCGCCTCTCTGAGTGCTCGAGTCCCCCCGACTACCTCAGTCCCCCTGGCTCGCCCTGCAGCCCTCAG
CCTCCGCCCTCCACGCAGGGGACCGGCGGCAGCTGTGTGAGCTCACCCGGGCCCAGCCGAATCGCCGACT
ACCTGCTGCTACCCCTAGCTGAGCGCGAGCATGTGTCCCGGGCACTGTGCATCCACACCGGCCGCGAGCT
GCGCTGCAAGGAATTCCCCATTAAACACTACCAGGACAAAATCAGGCCTTACATCCAGCTGCCGTCCCAT
AGCAACATCACTGGCATTGTGGAGGTGCTCCTTGGTGAGAGCAAGGCCTACGTCTTCTTCGAGAAGGACT
TTGGAGACATGCACTCCTATGTGCGGAGCCGAAAACGGCTTCGGGAAGAGGAGGCCGCTCGGCTCTTCAA
GCAGATTGTTTCCGCCGTCGCCCACTGCCACCAGTCGGCCATCGTGCTGGGGGACCTGAAGCTCAGGAAG
TTCGTCTTCTCCACCGAGGAGAGAACCCAGCTTAGACTGGAAAGCCTGGAAGATACTCACATCATTAAGG
GTGAAGATGATGCGCTGTCAGATAAACATGGCTGCCCAGCCTACGTGAGCCCCGAGATCCTCAACACTAC
TGGGACCTACTCTGGAAAGGCGGCGGACGTCTGGAGCCTCGGGGTGATGCTCTATACGCTTTTGGTCGGA
CGATACCCCTTTCATGACTCAGATCCGAGTGCCCTTTTTTCCAAAATCCGCCGTGGACAGTTCTGCATTC
CTGAGCACGTCTCCCCCAAAGCCAGGTGCCTCATCCGAAGCCTCCTAAGACGAGAACCTTCTGAGAGACT
CACAGCTCCTCAGATCTTACTCCATCCCTGGTTCGAGTATGTCTTGGAACCGGGCTATGTTGACTCAGAA
ATAGGAACTTCAGACCAGATTGTTCCTGAGTACCAGGAGGACAGTGACATAAGTTCCTTCTTCTGCTAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_144549
Insert Size 1119 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_144549.4, NP_653132.1
RefSeq Size 4026 bp
RefSeq ORF 1119 bp
Locus ID 211770
UniProt ID Q8K4K4
Cytogenetics 15 D1
Summary Adapter protein involved in protein degradation by interacting with COP1 ubiquitin ligase (PubMed:23515163, PubMed:20410507). Promotes CEBPA degradation and inhibits its function (PubMed:20410507). Controls macrophage, eosinophil and neutrophil differentiation via the COP1-binding domain (PubMed:24003916, PubMed:23515163). Regulates myeloid cell differentiation by altering the expression of CEBPA in a COP1-dependent manner (PubMed:23515163). Interacts with MAPK kinases and regulates activation of MAP kinases, but has no kinase activity (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_144549" in other vectors (4)
SKU Description Size Price
MG205755 Trib1 (tGFP-tagged) - Mouse tribbles homolog 1 (Drosophila) (Trib1) 10 ug
$489.00 $657.00
MR205755 Trib1 (Myc-DDK-tagged) - Mouse tribbles homolog 1 (Drosophila) (Trib1) 10 ug
$289.00 $457.00
MR205755L3 Lenti ORF clone of Trib1 (Myc-DDK-tagged) - Mouse tribbles homolog 1 (Drosophila) (Trib1) 10 ug
$757.00
MR205755L4 Lenti ORF clone of Trib1 (mGFP-tagged) - Mouse tribbles homolog 1 (Drosophila) (Trib1) 10 ug
$757.00

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