Ufd1 (NM_011672) Mouse Untagged Clone

CAT#: MC208050

Ufd1l (untagged) - Mouse ubiquitin fusion degradation 1 like (Ufd1l), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ufd1
• Synonyms: Ufd1; Ufd1l
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC208050 representing NM_011672
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTTCTTTCAACATGTTTGACCACCCGATTCCCCGGGTCTTCCAGAACCGCTTCTCCACGCAGTACC
  GCTGCTTCTCCGTGTCCATGCTAGCAGGGCCTAATGACAGGTCAGATGTGGAGAAAGGAGGGAAGATAAT
  TATGCCACCCTCAGCCCTCGATCAACTCAGCCGGCTCAACATTACCTATCCTATGCTGTTTAAATTGACC
  AATAAGAATTCAGATCGGATGACACACTGCGGTGTACTGGAGTTTGTTGCTGATGAAGGCATCTGTTACC
  TCCCCCACTGGATGATGCAGAATTTGCTGTTGGAGGAAGGGGGCCTGGTTCAGGTGGAAAGTGTCAACCT
  CCAAGTGGCGACCTACTCTAAGTTCCAGCCTCAGAGCCCAGACTTCCTGGATATTACCAACCCTAAAGCG
  GTATTAGAAAATGCATTGAGAAACTTCGCCTGTCTGACGACTGGAGATGTGATTGCTATCAACTACAATG
  AGAAGATCTATGAGCTGCGGGTGATGGAGACCAAACCTGACAAGGCTGTATCCATTATTGAATGTGACAT
  GAATGTGGATTTTGATGCTCCCTTGGGATACAAAGAACCAGAAAGACCAGTGCAGCATGAGGAGTCAATA
  GAGGGAGAAGCTGACCACAGTGGCTATGCCGGAGAGGTGGGCTTCCGTGCCTTCTCTGGTTCTGGGAATA
  GACTGGATGGGAAGAAAAAAGGGGTTGAGCCCAGTCCCTCCCCAATCAAGCCTGGAGACATCAAAAGAGG
  AATTCCTAATTACGAATTTAAGCTTGGTAAGATCACTTTCATCAGAAATTCACGTCCATTGGTCAAAAAG
  GTTGAAGAGGATGAAGCTGGAGGCAGATTCATTGCTTTCTCTGGAGAAGGACAGTCACTGCGTAAGAAGG
  GAAGAAAGCCCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011672
• Insert Size: 924 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011672.4, NP_035802.3
• RefSeq Size: 1996 bp
• RefSeq ORF: 924 bp
• Locus ID: 22230
• UniProt ID: P70362
• Cytogenetics: 16 11.65 cM
• Gene Summary: Essential component of the ubiquitin-dependent proteolytic pathway which degrades ubiquitin fusion proteins. The ternary complex containing UFD1, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. It may be involved in the development of some ectoderm-derived structures (By similarity). Acts as a negative regulator of type I interferon production via the complex formed with VCP and NPLOC4, which binds to DDX58/RIG-I and recruits RNF125 to promote ubiquitination and degradation of DDX58/RIG-I (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and is the protein-coding transcript.