Endov (NM_177394) Mouse Untagged Clone

CAT#: MC207963

Endov (untagged) - Mouse RIKEN cDNA A730011L01 gene (A730011L01Rik), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Endov
• Synonyms: A730011L01Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC207963 representing NM_177394
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTGGCCTGAAGGCCCCCTATGTGTCAGGCTTCCTGGCCTTCCGAGAGGTCCCTTTCCTGGTGGAGT
  TGGTACAGCGGCTGCAAGAGAAGGAACCAGATCTCATGCCCCAGGTCGTTCTTGTGGATGGAAACGGGGT
  GCTTCACCAACGAGGCTTCGGGGTGGCCTGCCACCTTGGTGTCCTTACAGAGCTGCCATGCATCGGGGTG
  GCCAAGAAGCTCCTGCAGGTGGATGGACTGGAGAACAATGCTCTGCACAAGGAGAAGATTGTGCTCCTGC
  AGGCCGGAGGAGACACATTTCCTCTGATAGGCAGCTCTGGGACTGTCCTGGGAATGGCCCTGAGGAGCCA
  TGACCACAGCACCAAGCCCCTCTATGTCTCTGTGGGCCACAGAATAAGCCTGGAGGTCGCTGTGCGCCTC
  ACCCACCACTGCTGTAGGTTCCGGATCCCAGAACCTATACGCCAGGCTGACATCCGCTCTCGAGAGTACA
  TCCGAAGGACTCTAGGGCAGCTTGGGGTGGCTCCTGCACAGAGAAAGGACAGGAGCCAGAAAGAGCAGAG
  GCCAAATGCATGCCCCCAAGGAGGCCCAGGAGCACTTGCAGATCAAGGCAGGCCTCCTGAATGCGACGGC
  AGAGACTCCAGCTCAGACCGGAAAGCCCCCGAGCCAGGCTTCCAGGAGCAGAAGGACCAGCAGTTGGAGG
  GAACCGGGCATCAGGAAGACTCGGACCTCTGGCCTCCTTCTCCAGCCTGGGTACAGTCACCACCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_177394
• Insert Size: 768 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177394.3, NP_796368.1
• RefSeq Size: 5164 bp
• RefSeq ORF: 768 bp
• Locus ID: 338371
• UniProt ID: Q8C9A2
• Cytogenetics: 11 E2
• Gene Summary: Endoribonuclease that specifically cleaves inosine-containing RNAs: cleaves RNA at the second phosphodiester bond 3' to inosine. Has strong preference for single-stranded RNAs (ssRNAs) toward double-stranded RNAs (dsRNAs). Cleaves mRNAs and tRNAs containing inosine. Also able to cleave structure-specific dsRNA substrates containing the specific sites 5'-IIUI-3' and 5'-UIUU-3'. Inosine is present in a number of RNAs following editing; the function of inosine-specific endoribonuclease is still unclear: it could either play a regulatory role in edited RNAs, or be involved in antiviral response by removing the hyperedited long viral dsRNA genome that has undergone A-to-I editing. Binds branched DNA structures (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) represents an alternate 5' splice pattern and uses a downstream start codon, compared to variant 1. Isoform 2 has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.