Rgp1 (NM_172866) Mouse Untagged Clone

CAT#: MC207931

Rgp1 (untagged) - Mouse RGP1 retrograde golgi transport homolog (S. cerevisiae) (Rgp1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rgp1
• Synonyms: 1110029E03Rik; AI527196; F730001J03; mKIAA0258
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC207931 representing NM_172866
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCGAAGTGGTAGCTGAGCTGAGCAGAGGTCCTGTGTTTCTGGCGGGGGAGGCTCTGGAATGTGTGG
  TGACTGTCACTAATCCCCTTCCCCCGACCGCCACTTCTGCATCCAGTGAGGCTCTGGCCTGGGCCAGTGC
  CCAGATCCACTGCCAGTTCCATGCCAGCGAGAGTCGAGTAGCACTGCCACCCCCTGACTCTAGTCAGCCG
  GACGTCCAGCCTGACAGCCAGACTGTCTTTCTGCCACACCGAGGTGAGAGGGGCCAGTGTATCCTTTCTA
  CTCCACCCAAAATTCTGTTTTGTGACCTGAGGCTAGACCCTGGAGAGTCCAAATCATATTCCTACAGTGA
  AGTGCTGCCCACGGAGGGCCCGCCTTCCTTTCGGGGTCAGTCAGTCAAATATGTCTACAAATTGACCATT
  GGCTGCCAGCGCGTGAACTCACCCATCACCTTACTCCGGGTTCCTCTGAGAGTTCTCGTGCTGACTGGTC
  TTCAAGATGTCCACTTCCCCCAGGATGAGGCTGTGGCGCCATCCAGTCCATTCTTAGAGGAGGATGACAG
  TGGGAAGAAGGACTCATGGCTGGCTGAGCTAGCTGGGGAGCGTCTCATGGCTGCCACATCCTGCCGCAGC
  CTCCATCTGTACAATATCAGCGATGGCCGAGGGAAAGTCGGGACGTTCGGTATCTTCAAATCTGTGTACA
  GACTTGGCGAAGACGTGGTGGGAACCTTAAATTTAGGGGAAGGAACCGTAGCTTGTCTGCAGTTTTCAGT
  AAGCTTGCAGACTGAAGAACGGGTACAGCCCGAATACCAGCGACGCCGCGGGACCGGAGTTGCCCCTTCA
  GTGTCCCATGTGACTCATGCCCGGCACCAGGAGTCCTGCCTACATACAACCAGAACCAGCTTCTCTCTCC
  CCATCCCTCTTTGCTCTACCCCTGGCTTCTGCACTGCCATTGTGTCCTTGAAGTGGCGATTACATTTTGA
  ATTTGTAACATCCCGAGAACCAGGATTGGTACTTCTGCCGCCATTGGAGCAGCCTGAGCCTGCGACCTGG
  ACAGGGCCTGAACAGGTGCCTGTAGACACCTTCAGCTGGGACCTCCCCATCAAAGTGCTTCCTACAAGCC
  CCACCCTGGTCTCATATGCTGCCCCAGGCCCTAGCACCAGCAGTATAACTATCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_172866
• Insert Size: 1176 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172866.3, NP_766454.1
• RefSeq Size: 5868 bp
• RefSeq ORF: 1176 bp
• Locus ID: 242406
• UniProt ID: Q8BHT7
• Cytogenetics: 4 A5
• Gene Summary: The RIC1-RGP1 complex acts as a guanine nucleotide exchange factor (GEF), which activates RAB6A by exchanging bound GDP for free GTP and may thereby required for efficient fusion of endosome-derived vesicles with the Golgi compartment. The RIC1-RGP1 complex participates in the recycling of mannose-6-phosphate receptors.[UniProtKB/Swiss-Prot Function]