Lim2 (NM_177693) Mouse Untagged Clone

CAT#: MC207914

Lim2 (untagged) - Mouse lens intrinsic membrane protein 2 (Lim2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Lim2
• Synonyms: 19kDa; 4833403J20; MP19; To3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC207914 representing NM_177693
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACAGCTTCATGGGTGGCGGCCTCTTCTGTGCCTGGGTGGGGACCATCCTGTTGGTGGTAGCCACAG
  CAACTGACCACTGGATGCAGTACCGGCTGTCGGGGTCCTTCGCACACCAGGGCCTGTGGCGTTACTGCCT
  GGGCAACAAGTGCTTCCTGCAGACCGAGAGCATCGCATATTGGAATGCCACCCGGGCTTTCATGATCCTG
  TCTGCCCTGTGTGCCACCTCGGGCATCATCATGGGTGTCCTAGCCTTTGCGCAGCAATCCACCTTCACCC
  GTCTCTCCAGGCCCTTCTCTGCCGGCATCATGTTTTTTGCCTCCACCCTTTTTGTCCTGTTGGCCTTGGC
  TATTTACACTGGAGTCACCGTCAGTTTCCTCGGCCGCCGCTTTGGAGACTGGCGCTTTTCATGGTCTTAC
  ATCCTGGGCTGGGTGGCCCTGCTCATGACCTTCTTTGCAGGAATTTTCTACATGTGTGCCTACCGGATGC
  ATGAGTGCCGGCGCCTAGCCACCCCACGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_177693
• Insert Size: 522 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177693.3, NP_808361.1
• RefSeq Size: 866 bp
• RefSeq ORF: 522 bp
• Locus ID: 233187
• UniProt ID: P56563
• Cytogenetics: 7 28.25 cM
• Gene Summary: Present in the thicker 16-17 nm junctions of mammalian lens fiber cells, where it may contribute to cell junctional organization. Acts as a receptor for calmodulin. May play an important role in both lens development and cataractogenesis.[UniProtKB/Swiss-Prot Function]