Ctdsp1 (NM_153088) Mouse Untagged Clone

CAT#: MC207898

Ctdsp1 (untagged) - Mouse CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase 1 (Ctdsp1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ctdsp1
• Synonyms: GIP; Nif3; NLIIF; SCP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC207898 representing NM_153088
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACAGCTCGGCCGTCATTACTCAGATCAGCAAGGAGGAGGCGCGGGGCCCGCTGCGGGGCAAAGGTG
  ACCAGAAGTCAGCAGTTTCCCAGAAGCCCCGGAGTCGGGGCATCCTCCACTCCCTCTTCTGCTGTGTCTG
  CCGGGATGATGGGGAGCCCCTACCTGCCCACAGTGGGGCTCCCCTGCTGGTGGAGGAAAATGGCGCCATC
  CCTAAGCACACCCCAGTCCAGTACCTGCTTCCCGAGGCCAAGGCCCAGGACTCAGACAAGATCTGCGTCG
  TCATCGACCTGGACGAGACCCTGGTGCACAGCTCCTTCAAGCCAGTGAACAATGCGGACTTCATCATCCC
  GGTGGAGATAGATGGAGTGGTCCACCAGGTCTATGTGCTGAAGCGGCCCCACGTAGATGAGTTCCTACAG
  CGAATGGGCGAGCTCTTTGAATGTGTGCTGTTCACTGCCAGCCTTGCCAAGTACGCAGACCCTGTAGCTG
  ATCTGCTGGACAAGTGGGGAGCCTTCCGTGCCAGGCTGTTTCGAGAGTCGTGTGTCTTCCACCGGGGGAA
  CTACGTAAAGGACCTGAGCAGGCTGGGCCGAGACCTGCGGCGAGTGCTCATTCTAGACAACTCACCCGCC
  TCCTATGTCTTCCATCCAGACAATGCCGTACCAGTGGCCTCGTGGTTTGACAACATGAGTGACACTGAGC
  TACACGATCTCTTACCCTTCTTTGAGCAACTCAGCCGCGTGGACGACGTATACTCAGTGCTCAGACAGCC
  TAGGCCCGGGAGCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_153088
• Insert Size: 786 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153088.2, NP_694728.1
• RefSeq Size: 2790 bp
• RefSeq ORF: 786 bp
• Locus ID: 227292
• UniProt ID: P58466
• Cytogenetics: 1 C3
• Gene Summary: Preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residue repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation (By similarity). Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells.[UniProtKB/Swiss-Prot Function]