Tmem55b (NM_001033271) Mouse Untagged Clone

CAT#: MC207885

Tmem55b (untagged) - Mouse transmembrane protein 55b (Tmem55b), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tmem55b
• Synonyms: BC039161; Pip4p1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC207885 representing NM_001033271
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCAGACGGAGAGCGCTCCCCGCTGCTCTCCGAGGCCGGCGATGGCGGCGCTGGTGGCAACGGCT
  TAGCGGGCCCGGGCGGGAGCGCGACCGGGCCCGGTGGAGGCCTGACGCCCTCCGCACCTCCGTACGGAGC
  CGGTAAACATGCCCCGCCCCAGGCATTTCCCCCGTTCCCTGAGGGACATCCAGCCGTGTTGCCTGGAGAG
  GACCCACCCCCCTACTCACCCTTGACTAGCCCGGATAGTGGGAGTGCCCCCATGATTACCTGTCGCGTCT
  GTCAGTCTCCGATCAACGTGGAAGGCAAGATGCATCAGCATGTAGTCAAATGTGGTGTCTGCAACGAAGC
  CACTCCCATCAAGAATGCACCTCCAGGAAAGAAGTATGTCCGATGCCCCTGTAATTGTCTCCTTATCTGC
  AAAGTGACTTCTCAGCGGATTGCCTGCCCTCGGCCCTACTGCAAAAGAATCATCAACCTAGGGCCTGTGC
  ATCCAGGACCTCTGAGTCCAGAGCCACAGCCAATGGGTGTCAGGGTCATCTGTGGACATTGCAAGAACAC
  GTTTCTGTGGACAGAATTCACAGACCGAACCTTGGCACGATGCCCTCACTGCAGAAAAGTGTCATCTATT
  GGGAGACGATATCCTAGGAAGAGATGTATTTGCTGCTTCTTGCTTGGTTTACTCTTGGCAGTCACTGCCA
  CTGGCCTTGCTTTTGGTACATGGAAGCATGCACAGCAATATGGAGGGATCTACGCAGCCTGGGCATTTGT
  CATTTTGTTGGCTGTTCTATGTTTGGGTCGGGCCCTTTATTGGGCTTGTATGAAGGTCAGCCATCCTGTC
  CAGAACTTCTCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001033271
• Insert Size: 855 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033271.5, NP_001028443.1
• RefSeq Size: 1623 bp
• RefSeq ORF: 855 bp
• Locus ID: 219024
• UniProt ID: Q3TWL2
• Cytogenetics: 14 C1
• Gene Summary: Catalyzes the hydrolysis of phosphatidylinositol-4,5-bisphosphate (PtdIns-4,5-P2) to phosphatidylinositol-4-phosphate (PtdIns-4-P) (By similarity). Does not hydrolyze phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-bisphosphate, inositol 3,5-bisphosphate, inositol 3,4-bisphosphate, phosphatidylinositol 5-monophosphate, phosphatidylinositol 4-monophosphate and phosphatidylinositol 3-monophosphate (By similarity). Regulates lysosomal positioning by recruiting JIP4 to lysosomal membranes, thus inducing retrograde transport of lysosomes along microtubules (By similarity). Contributes to assembly of the V-ATPase complex in lipid rafts of the lysosomal membrane and to subsequent amino acid-dependent activation of mTORC1 (PubMed:29644770). May play a role in the regulation of cellular cholesterol metabolism (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).