Ube2e2 (NM_144839) Mouse Untagged Clone

CAT#: MC207884

Ube2e2 (untagged) - Mouse ubiquitin-conjugating enzyme E2E 2 (UBC4/5 homolog, yeast) (Ube2e2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ube2e2
• Synonyms: BC016265
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC207884 representing NM_144839
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCACTGAGGCGCAGAGAGTTGATGACAGTCCAAGCACTAGTGGAGGAAGTTCTGATGGAGATCAGC
  GGGAGAGCGTTCAGCAGGAACCTGATCGAGAACAAGTTCAGCCTAAGAAAAAGGAGGGGAAGATATCCAG
  CAAGACAGCCGCGAAACTGTCAACCAGTGCCAAAAGAATTCAGAAGGAGCTTGCAGAAATCACGCTGGAT
  CCCCCTCCCAACTGTAGTGCTGGACCCAAAGGAGACAACATTTATGAATGGAGGTCAACTATATTGGGAC
  CCCCAGGATCTGTCTATGAAGGAGGGGTATTCTTTCTTGACATTACCTTTTCACCAGATTATCCGTTTAA
  ACCCCCTAAGGTCACCTTCCGAACGAGAATCTATCACTGTAATATCAACAGCCAGGGTGTAATCTGTCTG
  GACATCCTCAAGGACAACTGGAGTCCGGCCCTGACCATCTCTAAAGTCCTCCTGTCCATCTGCTCACTAC
  TTACAGACTGCAACCCTGCCGACCCTCTGGTGGGCAGTATTGCCACACAGTACATGACCAACAGAGCGGA
  GCATGACCGGATGGCCAGACAGTGGACCAAGCGGTACGCCACCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_144839
• Insert Size: 606 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144839.1, NP_659088.1
• RefSeq Size: 1410 bp
• RefSeq ORF: 606 bp
• Locus ID: 218793
• UniProt ID: Q91W82
• Cytogenetics: 14 A2
• Gene Summary: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. Catalyzes the ISGylation of influenza A virus NS1 protein.[UniProtKB/Swiss-Prot Function]