Tusc3 (NM_030254) Mouse Untagged Clone

CAT#: MC207781

Tusc3 (untagged) - Mouse tumor suppressor candidate 3 (Tusc3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tusc3
• Synonyms: AU022242; BC003311; N33
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC207781 representing NM_030254
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGCCAGGGCCGCTCCTTCACGCCGGAGACAGGCGGGCCGCCGGTTGCGCTACCTGCCTACTGGTA
  GCTTTCCCTTCCTCCTCTTGCTGCTGCTGCTCTGCATCCAGCTCGGGGGCGGACAGAAGAAAAAGGAGAA
  CCTTTTGGCTGAAAAAGTGGAGCAGCTGATGGAATGGAGTTCCAGGCGCTCGATCTTCAGAATGAACGGC
  GACAAGTTCCGGAAGTTTGTAAAAGCCCCACCTCGGAACTACTCCATGATCGTCATGTTCACTGCTCTGC
  AGCCTCAGCGGCAGTGTTCTGTGTGCAGGCAGGCTAACGAAGAATATCAAATCCTGGCTAATTCCTGGCG
  TTATTCATCTGCTTTTTGCAACAAACTGTTTTTTGGAATGGTGGACTATGATGAAGGGACAGATGTTTTT
  CAACAGCTCAACATGAACTCCGCTCCCACATTCATGCATTTTCCTTCAAAAGGCAGACCCAAGAGAGCTG
  ATACTTTTGACCTTCAACGAATTGGATTTGCAGCTGAGCAGCTAGCAAAATGGATTGCCGACAGGACGGA
  TGTTCATATTCGAGTTTTCCGGCCACCCAACTACTCAGGCACCATTGCTTTGGCCCTGTTAGTGTCACTG
  GTTGGTGGCTTGCTTTATCTAAGGAGGAACAACTTGGAGTTTATCTATAACAAGACTGGTTGGGCCATGG
  TATCTCTGTGTATAGTCTTTGCTATGACGTCTGGCCAGATGTGGAATCATATCCGTGGACCTCCATATGC
  TCATAAGAACCCACACAATGGACAAGTGAGCTACATTCATGGAAGCAGCCAGGCTCAGTTTGTGGCAGAG
  TCACACATCATTCTAGTACTGAATGCTGCTATCACCATGGGGATGGTTCTTCTAAATGAAGCAGCAACTT
  CCAAAGGGGATGTCGGGAAAAGACGCATCATTTGCCTCGTGGGATTGGGCTTGGTGGTCTTCTTCTTCAG
  TTTTCTCCTTTCAATATTTCGTTCAAAGTACCATGGCTATCCATATAGCTTTTTAATTAAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_030254
• Insert Size: 1044 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030254.3, NP_084530.3
• RefSeq Size: 1527 bp
• RefSeq ORF: 1044 bp
• Locus ID: 80286
• UniProt ID: Q8BTV1
• Cytogenetics: 8 A4
• Gene Summary: Acts as accessory component of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. Involved in N-glycosylation of STT3B-dependent substrates. Specifically required for the glycosylation of a subset of acceptor sites that are near cysteine residues; in this function seems to act redundantly with MAGT1. In its oxidized form proposed to form transient mixed disulfides with a glycoprotein substrate to facilitate access of STT3B to the unmodified acceptor site. Has also oxidoreductase-independent functions in the STT3B-containing OST complex possibly involving substrate recognition.[UniProtKB/Swiss-Prot Function]