Polr3h (NM_030229) Mouse Untagged Clone

CAT#: MC207778

Polr3h (untagged) - Mouse polymerase (RNA) III (DNA directed) polypeptide H (Polr3h), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Polr3h
• Synonyms: 5031409G22Rik; RPC8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC207778 representing NM_030229
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCGTGCTGGTGGAGATGGTGGACACCGTGCGCATACCCCCGTGGCAGTTTGAGCGGAAACTCAACG
  ATTCCATTGCTGAGGAGCTGAACAAGAAATTGGCCAACAAGGTCGTATACAACGTGGGACTCTGCATCTG
  TCTGTTTGATATCACCAAGCTGGAGGACGCCTATGTGTTCCCAGGGGATGGAGCATCCCACACCAAAGTC
  CATTTCCGCTACGTGGTGTTTCACCCCTTCCTGGATGAGATCCTCATTGGGAAGATCAAAGGCTGCAGCC
  CAGAGGGTGTGCACGTCTCTTTAGGGTTCTTTGATGACATTCTCATCCCCCCTGAGTCACTGCAGCAGCC
  TGCCAAGTTTGATGAAGCGGAGCAAGTGTGGGTGTGGGAGTACGAGACGGAGGAAGGAGCGCATGACCTG
  TACATGGACACTGGGGAGGAGATCCGCTTCAGGGTGGTGGATGAGAGCTTTGTGGACACATCCCCCACAG
  GACCCAGTTCCGCCGAGGCTGCCTCTTCAAGCGAGGAGCTGCCCAAGAAGGAAGCACCATATACACTTGT
  GGGATCTATCAGTGAGCCAGGACTAGGCCTTCTCTCCTGGTGGACGAGCAACTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_030229
• Insert Size: 615 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030229.4, NP_084505.2
• RefSeq Size: 2514 bp
• RefSeq ORF: 615 bp
• Locus ID: 78929
• UniProt ID: Q9D2C6
• Cytogenetics: 15 E1
• Gene Summary: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNA. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts induce type I interferon and NF- Kappa-B through the RIG-I pathway (By similarity).[UniProtKB/Swiss-Prot Function]