Noxo1 (NM_027988) Mouse Untagged Clone

CAT#: MC207709

Noxo1 (untagged) - Mouse NADPH oxidase organizer 1 (Noxo1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Noxo1
• Synonyms: 2310034C04Rik; hslt; P41NOX; P41NOXA; P41NOXB; P41NOXC; Snx28
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC207709 representing NM_027988
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAAGCCCAAGACACCCAGTATCAGCCCATGCTGTAGCCTTGGTGCAAATGGACCGACTCCAGACAT
  TTGCCTTCTCCGTGTGCTGGTCAGACAACAGTGACACATTTGTACGGAGGAGCTGGGATGAGTTCAGGCA
  GCTCCAGAAGACCCTTAAGAAAACCTTCCCAGTGGAGGCAGGCCTGCTACGGAGATCTGAACAAGTTCTT
  CCCAAGCTTCCTGATGCTCCATTGCTGACACGTCGGGGGCATACTGGTCGAGGACTGGTACGTTTGCGGC
  TGCTGGACACCTATGTACAGGCATTGCTGGCAACCTCAGAACACATATTGAGGAGTTCAGCACTTCACGG
  CTTCTTTGTACCCAAACCTCTGGATCTGGAGCCCATGCTGCCTCCTGGCAGCCTGGTGATCCTGCCTACA
  CCAGAGGAGCCCTTATCCCAACCCAGAGGCAGCCTTGACATTCACAGCCTGGAGGCTCAGAGCATTCCCT
  GTGTACAGCCTTTCCACACTCTTGACATAAGAGACAGACCTTTCCACACCAAGGCTCAAGAAATTCTGGA
  CATATTACTACGACATCCTTCAGGCTGGTGGCTGGTGGAGAACAAGGATCAGCAGGTAGCCTGGTTTCCA
  GCTCCCTACCTGGAGGAGGTAGCAACGTGCCAAGGCCAGGAGTCAGGCCTGGCTTTGCAAGGAAGTGGGA
  GGCAGTTCTGCACTACCCAGGCCTACGAGGGCAGTCGCTCTGATGAGCTATCCGTGCCCTCAGGGGCACG
  TGTCCATGTGCTGGAGACCTCAGACCGAGGCTGGTGGCTGTGCAGGTATAATGGCCGGACAGGCCTACTC
  CCTGCAATGTCGCTGCAACCTGAAGGGCTGGGCTCACTCCTGGGCAGGCCAGGGTTCCCAGACAGTGCTG
  GGGCAGACAAGGTGGCTGAGGACAGAACCATTCCCCCTGTAGTACCAACTCGTCCCTGTATGAGTGCCAT
  CCAGAGTCGATGCTGCTCCATTACCCGCAGGGCACTGGCACAGGAACAAGGGACTCGGGTTCCCCGTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_027988
• Insert Size: 1050 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC019525, AAH19525
• RefSeq Size: 2239 bp
• RefSeq ORF: 1050 bp
• Locus ID: 71893
• UniProt ID: Q8VCM2
• Cytogenetics: 17 12.49 cM
• Gene Summary: Constitutively potentiates the superoxide-generating activity of NOX1 and NOX3 and is required for the biogenesis of otoconia/otolith, which are crystalline structures of the inner ear involved in the perception of gravity. Isoform 3 is more potent than isoform 1 in activating NOX3. Together with NOXA1, may also substitute to NCF1/p47phox and NCF2/p67phox in supporting the phagocyte NOX2/gp91phox superoxide-generating activity.[UniProtKB/Swiss-Prot Function]