Ubr7 (NM_025666) Mouse Untagged Clone

CAT#: MC207589

Ubr7 (untagged) - Mouse ubiquitin protein ligase E3 component n-recognin 7 (putative) (Ubr7), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ubr7
• Synonyms: 5730410I19Rik; AA589405; AW557761
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC207589 representing NM_025666
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGAGCCGAGAGCCCCACCGAGTGCCAGGCGGAGCTGGAGCCCGTGGTGTCGCTGGTGGACGTGC
  TCGAGGAAGACGAGGAGTTGGAGAATGAGGCGTGCGCCGTCCTGGGCGGCAGCGACTCGGAGAAGTGCTC
  CTACTCGCAGGGCTCAGTGGGGAGACAAGCACTGTACGCCTGCAGCACCTGCACGCCAGAGGGAGAAGAG
  CCCGCAGGGATTTGTCTAGCTTGCAGTTACGAATGTCATGGAAGCCATAAACTGTTTGAACTATACACAA
  AAAGAAATTTTCGTTGTGATTGTGGAAACAGCAAGTTTAAAAACTTGGAATGCAAATTATTTCCTGACAA
  ATCAAAGGTAAATTCTTGCAATAAGTACAATGACAACTTTTTTGGATTATACTGCGTTTGTAAGAGACCT
  TATCCTGATCCTGAAGATGAGGTTCCAGATGAGATGATACAGTGTGTGGTCTGTGAAGACTGGTTCCATG
  GCAGGCATCTTGGTGCCATTCCCCCTGAGAGTGGGGATTTCCAGGAGATGGTGTGCCAGGCTTGTATGAG
  GCGCTGTTCTTTCTTGTGGGCTTATGCTGCCCAGTTGGCAGTCACCAGGATATCTGCTGAAGATGATGGG
  CTGCTGCCAAATGCTACTGGCATGGGAGATGAAGATGTTAGCAAGCCTGAAAATGGAGCTCCCCAAGATA
  ATGGCCTGAAAGAGGATGCTCCCGAGCATGGCAGGGATTCTGTCAACGAGGTGAAAGCTGAGCAGAAGAA
  CGAGCCCTGCAGCAGCTCTAGTTCTGAGTCTGACCTCCAGACAGTGTTTAAGAAAGAAAATATCAAAACT
  GAGCCACAGTCCAGCTGCAGACTTCAGGAACTCCAAGCCAAGCAGTTTGTAAAGAAAGATGCTGCCACCT
  ATTGGCCCTTGAACTGGCGCAGCAAACTATGCACCTGTCAAGACTGTATGAAAATGTATGGAGAACTAGA
  CGTCCTGTTCCTGACAGATGAATGTGATACAGTTCTGGCTTATGAGAACAAAGGCAAGAACGACCAGGCA
  ACTGATAGAAGGGACCCTTTAATGGACACCCTTAGCAGCATGAATAGAGTCCAGCAAGTGGAGCTCATCT
  GTGAATACAATGACTTGAAGACTGAACTTAAGGACTACCTGAAGAGATTTGCTGATGAAGGCACGGTTGT
  TAAGAGAGAAGACATTCAGCAGTTCTTTGAAGAGTTTCAATCAAAAAAGAGAAGGCGAGTGGACGGGTTA
  CAGTATTACTGCAGCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_025666
• Insert Size: 1278 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025666.2, NP_079942.1
• RefSeq Size: 3247 bp
• RefSeq ORF: 1278 bp
• Locus ID: 66622
• UniProt ID: Q8BU04
• Cytogenetics: 12 E
• Gene Summary: E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation.[UniProtKB/Swiss-Prot Function]