Mettl9 (NM_021554) Mouse Untagged Clone

CAT#: MC207545

Mettl9 (untagged) - Mouse methyltransferase like 9 (Mettl9), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mettl9
• Synonyms: 0610012D09Rik; AA517660; Drev; MNCb-5680
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC207545 representing NM_021554
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGACTGTTGGCGGGCTGGCTGTGCCTGAGCCTGGCGTCCGTGTGGCTGGCGCGGAGGATGTGGACGC
  TGCGGAGCCCGCTCTCCCGCTCCCTGTACGTGAACATGACTAGCGGCCCCGGCGGGCCAGCGGCGGCCGC
  GGGCGGCGGGAAGGACACGCACCAGTGGTATGTGTGCAACAGAGAGAAATTATGCGAATCACTTCAGTCT
  GTCTTTGTTCAGAGTTATCTTGACCAAGGAACACAGATCTTCTTAAACAACAGCATTGAGAAATCTGGCT
  GGCTATTTATCCAACTCTATCATTCTTTTGTATCATCTGTTTTTAGCCTGTTTATGTCTAGAACATCTAT
  TAACGGGTTGCTAGGAAGAGGCTCCATGTTTGTGTTCTCACCAGATCAGTTTCAGAGACTGCTTAGAATT
  AATCCGGACTGGAAAACCCATAGACTTCTTGATTTAGGTGCTGGAGATGGAGAAGTCACGAAAATCATGA
  GCCCTCATTTTGAAGAAATTTACGCCACTGAGCTTTCTGAAACAATGATCTGGCAGCTCCAGAAGAAGAA
  ATACAGAGTGCTTGGTATAAATGAATGGCAGAATACAGGGTTCCAGTATGATGTCATCAGCTGCTTAAAT
  TTGCTGGATCGCTGTGATCAGCCCCTGACATTGTTAAAAGATATCAGAAGTGTCTTGGAGCCCACCCAAG
  GCAGGGTCATCCTGGCATTGGTTTTGCCCTTTCATCCCTATGTAGAAAACGTAGGTGGCAAGTGGGAGAA
  ACCATCAGAAATTCTGGAAATCAAGGGACAGAATTGGGAAGAGCAAGTGAATAGCCTGCCTGAGGTGTTC
  AGAAAAGCCGGCTTTGTCGTCGAAGCTTTCACTAGACTGCCATACCTGTGTGAAGGCGACATGTACAATG
  ACTACTATGTTCTGGACGATGCTGTCTTTGTTCTCAGACCAGTATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_021554
• Insert Size: 957 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021554.2, NP_067529.2
• RefSeq Size: 1850 bp
• RefSeq ORF: 957 bp
• Locus ID: 59052
• UniProt ID: Q9EPL4
• Cytogenetics: 7 F2
• Gene Summary: Protein-histidine N-methyltransferase that specifically catalyzes 1-methylhistidine (pros-methylhistidine) methylation of target proteins (PubMed:33563959). Mediates methylation of proteins with a His-x-His (HxH) motif (where 'x' is preferably a small amino acid) (PubMed:33563959). Catalyzes methylation of target proteins such as S100A9, NDUFB3, SLC39A5, SLC39A7, ARMC6 and DNAJB12; 1-methylhistidine modification may affect the binding of zinc and other metals to its target proteins (PubMed:33563959). Constitutes the main methyltransferase for the 1-methylhistidine modification in cell (PubMed:33563959).[UniProtKB/Swiss-Prot Function]