Maea (NM_021500) Mouse Untagged Clone

CAT#: MC207544

Maea (untagged) - Mouse macrophage erythroblast attacher (Maea), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Maea
• Synonyms: 1110030D19Rik; EMP; Gid9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC207544 representing NM_021500
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGTGCAGGAGTCGGCGGCCCAGCTGTCCATGACCCTGAAGGTGCAGGAGTACCCGACCCTCAAGG
  TGCCCTATGAGACACTGAACAAACGCTTCCGAGCTGCTCAGAAAAACATCGATCGAGAGACTAGCCACGT
  CACCATGGTGGTAGCTGAGCTTGAGAAGACCTTGAGTAGTTGCCCAGCTGTGGACTCTGTGGTCAGCCTA
  TTGGATGGTGTGGTGGAGAAGCTGAGTGTCCTCAAGAGGAAGGCAGTAGAGTCCATCCAGGCCGAGGATG
  AGAGCGCCAAGCTCTGCAAACGTAGGATCGAGCACCTCAAGGAGCACAGCAGTGACCAGCCAGCAGCAGC
  CAGCATGTGGAAGCGGAAGCGCATGGACCGGATGATGGTGGAGCATCTGCTACGCTGTGGCTACTACAAC
  ACAGCTGTGAAGCTGGCTCGCCAGAGTGGCATCGAGGACCTTGTGAATATCGAGATGTTCCTGACAGCCA
  AAGAAGTGGAGGAGTCCTTGGAGAGGCGTGAGACAGCCACCTGCCTTGCCTGGTGCCATGATAACAAGTC
  CCGACTCCGGAAGATGAAGAGCTGCCTAGAGTTCAGCCTCAGGATTCAGGAGTTCATTGAACTTGTCCGG
  CAGAACAAGCGCCTGGATGCTGTGAGACATGCAAGAAAGCACTTCAGTCAGGCTGAAGGGAGCCAGCTGG
  ATGAGGTCCGCCAGGTCATGGGCATGTTGGCCTTCCCACCAGACACACATATCTCCCCATACAAGGACCT
  CCTGGACCCAGCCCGGTGGCGAATGCTGATCCAGCAGTTTCGATATGATAACTACCGGCTGCACCAGCTG
  GGAAACAGCTCAGTCTTCACCCTCACCCTGCAGGCTGGGCTCTCAGCAATAAAGACACCACAGTGCTACA
  AGGAGGATGGCAGCTCTAAGAGCCCTGACTGCCCTGTGTGCAGCCGCTCTCTGAACAAACTGGCACAGCC
  CCTGCCCATGGCTCACTGTGCCAACTCCCGCCTGGTCTGCAAGATCTCTGGTGACGTGATGAATGAGAAT
  AACCCACCCATGATGCTGCCTAATGGCTATGTCTATGGCTACAATTCTCTGCTTTCTATTCGTCAAGATG
  ATAAAGTTGTTTGCCCAAGAACCAAAGAAGTCTTCCACTTCTCCCAAGCTGAGAAAGTATACATCATGTA
  G
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_021500
• Insert Size: 1191 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021500.2, NP_067475.2
• RefSeq Size: 2128 bp
• RefSeq ORF: 1191 bp
• Locus ID: 59003
• UniProt ID: Q4VC33
• Cytogenetics: 5 B1
• Gene Summary: Core component of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1. MAEA and RMND5A are both required for catalytic activity of the CTLH E3 ubiquitin-protein ligase complex. MAEA is required for normal cell proliferation. The CTLH E3 ubiquitin-protein ligase complex is not required for the degradation of enzymes involved in gluconeogenesis, such as FBP1 (By similarity). Plays a role in erythroblast enucleation during erythrocyte maturation and in the development of mature macrophages (PubMed:16707498). Mediates the attachment of erythroid cell to mature macrophages; this MAEA-mediated contact inhibits erythroid cell apoptosis (By similarity). Participates in erythroblastic island formation, which is the functional unit of definitive erythropoiesis (PubMed:16707498, PubMed:17071116). Associates with F-actin to regulate actin distribution in erythroblasts and macrophages (PubMed:16707498). May contribute to nuclear architecture and cells division events (By similarity).[UniProtKB/Swiss-Prot Function]