Elovl2 (NM_019423) Mouse Untagged Clone

CAT#: MC207505

Elovl2 (untagged) - Mouse elongation of very long chain fatty acids (FEN1/Elo2, SUR4/Elo3, yeast)-like 2 (Elovl2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Elovl2
• Synonyms: AI317360; Ssc2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC207505 representing NM_019423
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCAGCTGAAGGCCTTTGATAATGAAGTCAATGCTTTCTTGGACAACATGTTTGGACCACGAGATT
  CTCGAGTTCGCGGGTGGTTCCTGCTGGACTCTTACCTTCCCACCTTCATCCTCACCATCACGTACCTGCT
  CTCGATATGGCTGGGTAACAAGTACATGAAGAACAGGCCTGCTCTGTCTCTCAGGGGCATCCTCACCTTG
  TATAACCTCGCAATCACACTTCTTTCTGCGTATATGCTGGTGGAGCTCATCCTCTCCAGCTGGGAAGGAG
  GTTACAACTTGCAGTGTCAGAATCTCGACAGTGCAGGAGAAGGTGATGTCCGGGTAGCCAAGGTCTTGTG
  GTGGTACTACTTCTCCAAACTAGTGGAGTTCCTGGACACGATTTTCTTTGTTCTACGAAAAAAGACCAAT
  CAGATCACCTTCCTTCATGTCTATCACCACGCGTCCATGTTCAACATCTGGTGGTGTGTTTTGAACTGGA
  TACCTTGTGGTCAAAGCTTCTTTGGACCCACCCTGAACAGCTTTATCCACATTCTCATGTACTCCTACTA
  CGGCCTGTCTGTGTTCCCGTCCATGCACAAGTACCTTTGGTGGAAGAAGTACCTCACACAGGCTCAGCTG
  GTGCAGTTCGTACTCACCATCACGCACACGCTGAGTGCCGTGGTGAAGCCCTGTGGCTTCCCCTTTGGCT
  GTCTCATCTTCCAGTCTTCCTATATGATGACGCTGGTCATCCTGTTCTTAAACTTCTATATTCAGACATA
  CCGGAAAAAGCCAGTGAAGAAAGAGCTGCAAGAGAAAGAAGTGAAGAATGGTTTCCCCAAAGCCCACTTA
  ATTGTGGCTAATGGCATGACGGACAAGAAGGCTCAATAA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_019423
• Insert Size: 879 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019423.2, NP_062296.1
• RefSeq Size: 3837 bp
• RefSeq ORF: 879 bp
• Locus ID: 54326
• UniProt ID: Q9JLJ4
• Cytogenetics: 13 A3.3
• Gene Summary: Catalyzes the first and rate-limiting reaction of the four reactions that constitute the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process allows the addition of 2 carbons to the chain of long- and very long-chain fatty acids (VLCFAs) per cycle. Condensing enzyme that catalyzes the synthesis of polyunsaturated very long chain fatty acid (C20- and C22-PUFA), acting specifically toward polyunsaturated acyl-CoA with the higher activity toward C20:4(n-6) acyl-CoA. May participate in the production of polyunsaturated VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators. Essential for the formation of C24:5(n-6) up to C30:5(n-6) PUFAs in testis, these fatty acids being indispensable for normal spermatogenesis and fertility.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (1).