Nit2 (NM_023175) Mouse Untagged Clone

CAT#: MC207496

Nit2 (untagged) - Mouse nitrilase family, member 2 (Nit2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nit2
• Synonyms: 1190017B19Rik; D16Ertd502e
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC207496 representing NM_023175
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTACTTTCCGCCTGGCCCTCATACAGCTTCAAGTTTCTTCCATTAAATCAGATAACCTTACCCGGG
  CTTGTAGCCTAGTGCGGGAGGCAGCAAAGCAAGGTGCCAACATAGTTTCTCTGCCTGAGTGCTTCAATTC
  TCCATATGGAACAACCTACTTTCCTGACTATGCAGAGAAGATTCCTGGAGAGTCCACACAAAAGCTTTCT
  GAAGTAGCAAAGGAGAGCAGCATATATCTCATTGGAGGCTCCATCCCTGAAGAGGATGCTGGGAAACTGT
  ATAATACCTGCTCTGTGTTTGGGCCTGATGGAAGTTTACTGGTAAAGCACAGGAAGATCCATCTGTTTGA
  CATTGATGTTCCTGGGAAAATTACGTTTCAAGAATCTAAAACATTGAGCCCTGGTGATAGTTTCTCCACA
  TTTGATACGCCTTACTGCAAAGTGGGCCTGGGCATCTGCTATGATATGCGCTTCGCGGAGCTTGCACAAA
  TCTATGCACAAAGAGGCTGCCAGCTCTTGGTGTATCCTGGAGCTTTCAATCTGACCACAGGACCAGCCCA
  CTGGGAGCTGCTTCAGCGAGCCCGGGCTGTTGATAATCAGGTGTATGTGGCTACAGCCTCTCCTGCTCGG
  GATGACAAAGCCTCGTATGTGGCCTGGGGACACAGCACTGTTGTGGATCCTTGGGGGCAGGTCCTAACCA
  AAGCTGGCACGGAGGAAACAATCCTGTACTCAGACATAGACCTGAAGAAGCTGGCTGAAATTCGGCAGCA
  AATCCCCATTTTAAAACAGAAACGAGCAGACCTCTATACAGTGGAATCAAAGAAGCCTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_023175
• Insert Size: 831 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023175.1, NP_075664.1
• RefSeq Size: 1292 bp
• RefSeq ORF: 831 bp
• Locus ID: 52633
• UniProt ID: Q9JHW2
• Cytogenetics: 16 34.22 cM
• Gene Summary: Has a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting 2-oxoglutaramate and 2-oxosuccinamate to biologically useful 2-oxoglutarate and oxaloacetate, respectively.[UniProtKB/Swiss-Prot Function]