Rnf13 (NM_001113413) Mouse Untagged Clone

CAT#: MC207454

Rnf13 (untagged) - Mouse ring finger protein 13 (Rnf13), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rnf13
• Synonyms: 2010001H16Rik; Rz; Rzf
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC207454 representing NM_001113413
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCTCTCCATTGGGATGCTCATGCTGTCCGCCACACAGGTCTACACCATCTTGACTGTGCAGCTCT
  TTGCATTCCTAAACCTGCTGCCTGTAGAAGCAGACATTTTAGCATATAATTTTGAAAATGCATCTCAGAC
  ATTTGAGGACCTTCCAGCAAGATTTGGTTATAGACTACCAGCTGAAGGCTTAAAGGGTTTTCTGATTAAC
  TCAAAACCAGAGAATGCGTGTGAACCCATAGTGCCTCCACCACTGAAAGACAATTCTTCCGGCACGTTCA
  TCGTGCTGATTAGAAGACTTGATTGCAATTTTGATATAAAGGTTTTAAATGCACAGAGAGCAGGATACAA
  AGCAGCCATAGTTCACAATGTAGACTCTGATGACCTGATTAGCATGGGATCCAACGACATTGATACACTA
  AAGAAAATTGACATTCCGTCTGTCTTTATTGGTGAATCATCAGCTAATTCCCTGAAAGATGAATTCACAT
  ATGAAAAAGGGGGCCACATTATCTTAGTTCCAGAACTGAGTCTTCCTTTGGAGTACTATCTGATCCCCTT
  CCTCATCATAGTGGGCATCTGCCTCATCTTAATAGTCATTTTCATGATCACAAAATTTGTTCAGGACAGA
  CATAGAAACAGAAGAAATAGACTTCGTAAAGATCAACTTAAAAAACTTCCCGTACATAAATTTAAAAAGG
  GAGATGAGTACGATGTGTGTGCTATCTGTCTGGAGGAGTATGAAGACGGAGATAAGCTAAGGATCCTCCC
  CTGCTCCCATGCTTATCATTGCAAGTGTGTAGATCCCTGGCTAACTAAAACCAAAAAGACCTGTCCAGTC
  TGCAAACAAAAGGTTGTTCCTTCCCAAGGTGACTCGGACTCTGACACAGACAGCAGTCAGGAAGAAAACC
  AAGTGTCAGAGCACACCCCTTTACTTCCACCCTCAGCTTCTGCCAGGACCCAGTCATTTGGGTCTCTGTC
  AGAATCCCACTCACATCACAACATGACAGAGTCTTCAGACTACGAGGACGACGACAATGAAGAGACCGAC
  AGCAGTGACGCAGACAACGAAATTACCGACCACAGTGTTGTGGTCCAGCTGCAGCCGAACGGTGAGCAGG
  ATTACAACATAGCAAATACTGTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001113413
• Insert Size: 1146 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001113413.2, NP_001106884.1
• RefSeq Size: 2872 bp
• RefSeq ORF: 1146 bp
• Locus ID: 24017
• UniProt ID: O54965
• Cytogenetics: 3 D
• Gene Summary: This gene encodes a member of the PA-TM-RING family of proteins that contain a protease associated (PA) domain and a RING finger domain separated by a transmembrane (TM) domain. The encoded protein is an E3 ubiquitin ligase localized to the endosomal-lysosomal vesicles and inner nuclear membrane. Mice lacking the encoded protein have impaired learning abilities associated with a decreased synaptic vesicle density and dysregulated SNARE complex assembly. Alternative splicing of this gene results in multiple transcript variants. A pseudogene for this gene has been identified on the X chromosome. [provided by RefSeq, Jan 2015]